FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37441
Sequences flagged as poor quality0
Sequence length281
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA5031.3434470233166849No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4921.3140674661467375No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4311.1511444672952111No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4261.137790123127053No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA4101.095056221788948No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3831.0229427632808952No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3801.0149301567800006No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3610.9641836489410005No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3580.9561710424401058No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3350.8947410592665794No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3250.8680323709302636No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3140.8386528137603162No Hit
AGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA3060.8172858630912636No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2980.795918912422211No Hit
CGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2710.7238054539141583No Hit
TGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2690.7184637162468951No Hit
CGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2610.6970967655778424No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2300.6142998317352635No Hit
TGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2290.6116289629016318No Hit
TGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2190.5849202745653161No Hit
TGACTACTAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2150.5742367992307899No Hit
TGACTACTCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2080.5555407173953687No Hit
TGACTACAAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2020.5395155043935792No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA2020.5395155043935792No Hit
TGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1980.5288320290590529No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1980.5288320290590529No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1980.5288320290590529No Hit
GGACTACTCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1960.5234902913917897No Hit
TGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1940.5181485537245266No Hit
GGACTACTAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1890.5047942095563687No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1880.5021233407227371No Hit
GGACTACTGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1860.4967816030554739No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1840.4914398653882108No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1780.47541465238642133No Hit
TGACTACACGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1780.47541465238642133No Hit
AGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1770.4727437835527897No Hit
TGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1750.46740204588552653No Hit
TGACTACCAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1730.4620603082182634No Hit
TGACTACGCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1730.4620603082182634No Hit
TGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1710.45671857055100024No Hit
TGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1710.45671857055100024No Hit
TGACTACTGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1700.45404770171736863No Hit
GGACTACGGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1690.4513768328837371No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1690.4513768328837371No Hit
GGACTACGAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1630.43535161988194754No Hit
GGACTACGCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1630.43535161988194754No Hit
TGACTACGAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1600.4273390133810529No Hit
GGACTACAAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1590.42466814454742124No Hit
CGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1520.4059720627120002No Hit
TGAATACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1510.40330119387836866No Hit
AGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1490.3979594562111055No Hit
TGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1490.3979594562111055No Hit
AGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1480.3952885873774739No Hit
TGACTACAGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1470.39261771854384225No Hit
TGACTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1430.38193424320931596No Hit
GGAATACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1430.38193424320931596No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1430.38193424320931596No Hit
CGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1420.3792633743756844No Hit
TGACTACCCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1420.3792633743756844No Hit
CGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1410.3765925055420528No Hit
CGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1410.3765925055420528No Hit
AGACTACTAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1380.3685798990411581No Hit
GGACTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1370.3659090302075265No Hit
AGAATACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1360.363238161373895No Hit
GGACTACCAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1360.363238161373895No Hit
AGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1320.3525546860393686No Hit
AGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1320.3525546860393686No Hit
CGACTACTAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1300.3472129483721055No Hit
GGACTACACGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1300.3472129483721055No Hit
GGACTACAGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1300.3472129483721055No Hit
CGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1260.33652947303757913No Hit
CGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1260.33652947303757913No Hit
CGAATACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1230.3285168665366844No Hit
AGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1210.32317512886942124No Hit
CGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1200.32050426003578963No Hit
CGACTACAAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1190.31783339120215803No Hit
AGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1190.31783339120215803No Hit
AGACTACTCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1190.31783339120215803No Hit
CGACTACTGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1180.3151625223685265No Hit
CGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1180.3151625223685265No Hit
TGACTACGGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1170.3124916535348949No Hit
AGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1150.30714991586763174No Hit
AGACTACTGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1150.30714991586763174No Hit
AGACTACGAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1140.3044790470340002No Hit
GGACTACCCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1130.3018081782003686No Hit
CGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1130.3018081782003686No Hit
AGACTACAGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1130.3018081782003686No Hit
AGACTACAAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1130.3018081782003686No Hit
CGACTACTCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1100.29379557169947385No Hit
CGACTACGAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1090.29112470286584224No Hit
AGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1090.29112470286584224No Hit
CGACTACAGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1080.2884538340322107No Hit
AGACTACCAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1050.28044122753131595No Hit
CGACTACCAGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1040.27777035869768435No Hit
CGACTACACGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA1040.27777035869768435No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1020.2724286210304212No Hit
AGACTACACGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA980.26174514569589485No Hit
CGACTACGGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA970.2590742768622633No Hit
AGACTACCCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA960.2564034080286317No Hit
AGACTACGCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA960.2564034080286317No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA890.23770732619321067No Hit
CGACTACCCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA870.23236558852594752No Hit
CGACTACGCGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA830.22168211319142117No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA790.21099863785689488No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.20832776902326325No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA770.20565690018963168No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA760.2029860313560001No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA760.2029860313560001No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA760.2029860313560001No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA740.19764429368873696No Hit
AGACTACGGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA740.19764429368873696No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.1923025560214738No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.1896316871878422No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA710.1896316871878422No Hit
CGACTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA710.1896316871878422No Hit
AGACTACCGGGGTCTCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCA700.18696081835421063No Hit
AGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA660.1762773430196843No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.17093560535242114No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA630.16826473651878956No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.165593867685158No Hit
CGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA610.16292299885152642No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.15491039235063167No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA550.14689778584973692No Hit
CGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA530.14155604818247375No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA500.133543441681579No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.13087257284794743No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA480.12820170401431585No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.12553083518068428No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.12553083518068428No Hit
GGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA460.1228599663470527No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.12018909751342112No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.12018909751342112No Hit
TGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA450.12018909751342112No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.11751822867978955No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.11484735984615795No Hit
GGACTACCGGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA420.11217649101252637No Hit
TGACTACGCGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA420.11217649101252637No Hit
TGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA420.11217649101252637No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA420.11217649101252637No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA420.11217649101252637No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA410.1095056221788948No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.10683475334526323No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA400.10683475334526323No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.10683475334526323No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA400.10683475334526323No Hit
TGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA400.10683475334526323No Hit
TGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA390.10416388451163162No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA390.10416388451163162No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.10149301567800005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)