FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42632
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA15663.673297053856258No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA12893.023550384687559No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5361.2572715331206605No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4661.093075623944455No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA4451.043816851191593No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4381.0273972602739725No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4090.959373240758116No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4050.9499906173766184No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3770.8843122537061363No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3660.8585100394070183No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3660.8585100394070183No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3470.8139425783449052No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3350.7857947082004128No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3350.7857947082004128No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.7811033965096641No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3150.7388815912929255No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3080.722462000375305No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3060.7177706886845562No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2910.6825858510039408No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2900.6802401951585663No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2840.6661662600863202No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2820.6614749483955714No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2820.6614749483955714No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2800.6567836367048226No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2800.6567836367048226No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2780.652092325014074No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2750.6450553574779508No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2740.6427097016325765No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.6380183899418277No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2700.633327078251079No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2650.6215987990242071No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2640.6192531431788328No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2610.6122161756427097No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2490.5840683054982173No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2480.5817226496528429No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.5793769938074685No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.5653030587352224No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.5559204353537249No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2320.544192156126853No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2310.5418465002814787No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2310.5418465002814787No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2250.5277725652092325No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.5230812535184838No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2220.5207355976731094No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2220.5207355976731094No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2210.5183899418277351No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2190.5136986301369862No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2190.5136986301369862No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2170.5090073184462376No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2170.5090073184462376No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.5043160067554888No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2140.5019703509101144No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2120.4972790392193657No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.4855507599924939No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2050.4808594483017452No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.4808594483017452No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2030.47616813661099644No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2000.46913116907487334No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2000.46913116907487334No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.45740288984800154No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.4456746106211297No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1800.422218052167386No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1780.4175267404766373No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.4034528054043911No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.4034528054043911No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.4034528054043911No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1660.3893788703321449No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1660.3893788703321449No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.37530493525989866No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1580.37061362356914995No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.3635766560330268No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1520.35653968849690376No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.3541940326515293No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1490.3495027209607806No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1490.3495027209607806No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1440.3377744417339088No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.3354287858885344No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1420.3330831300431601No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.3283918183524113No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.3237005066616626No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.31900919497091385No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.30493525989866765No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1290.3025896040532933No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1280.30024394820791894No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.29086132482642146No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1230.28851566898104714No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1120.262713454681929No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1100.2580221429911803No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.2580221429911803No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.2533308313004316No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1060.24863951960968286No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1030.24160255207355974No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1030.24160255207355974No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA980.2298742728466879No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA970.22752861700131355No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA910.21345468192906736No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.211109026083693No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA890.20876337023831865No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA890.20876337023831865No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA860.20172640270219555No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA850.19938074685682117No Hit
CCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA830.19468943516607246No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA830.19468943516607246No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.19234377932069807No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA820.19234377932069807No Hit
TCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA780.18296115593920062No Hit
GCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA780.18296115593920062No Hit
ACCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA770.18061550009382624No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA760.17826984424845188No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA750.1759241884030775No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA720.1688872208669544No Hit
GCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA710.16654156502158005No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.1618502533308313No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA670.15715894164008257No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA670.15715894164008257No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA670.15715894164008257No Hit
TCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA670.15715894164008257No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA650.15246762994933383No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA650.15246762994933383No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA640.15012197410395947No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA640.15012197410395947No Hit
ACCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA630.1477763182585851No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA630.1477763182585851No Hit
GCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA630.1477763182585851No Hit
ACCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA620.14543066241321073No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA610.14308500656783635No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA600.140739350722462No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.13839369487708764No Hit
ACCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA580.13604803903171325No Hit
TCCTATGGGGCGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA580.13604803903171325No Hit
CCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA570.1337023831863389No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.12901107149559016No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.12431975980484143No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA520.12197410395946708No Hit
CCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA520.12197410395946708No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.11962844811409269No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGCGGAAGCCTGA510.11962844811409269No Hit
CCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA500.11728279226871834No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA500.11728279226871834No Hit
CCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA500.11728279226871834No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA470.11024582473259524No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA470.11024582473259524No Hit
ACCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA470.11024582473259524No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.11024582473259524No Hit
CCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA470.11024582473259524No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.10790016888722086No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA460.10790016888722086No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.10790016888722086No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.10790016888722086No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.1055545130418465No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGA450.1055545130418465No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA450.1055545130418465No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA440.10320885719647213No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.10320885719647213No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.10320885719647213No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.10320885719647213No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.10086320135109778No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA430.10086320135109778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)