FastQCFastQC Report
Tue 7 May 2024
EGAF00008092035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26845
Total Bases8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA18006.705159247532129No Hit
TCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA6892.566585956416465No Hit
GCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA5341.9891972434345313No Hit
TCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4471.6651145464704789No Hit
ACCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4461.6613894579996273No Hit
TCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4421.6464891041162228No Hit
ACCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4281.5943378655243063No Hit
TCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4231.5757124231700503No Hit
ACCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4061.5123859191655802No Hit
GCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA4051.5086608306947291No Hit
CCCTATGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3741.3931830880983422No Hit
GCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3711.3820078226857888No Hit
TCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3611.344756937977277No Hit
TCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3611.344756937977277No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3561.326131495623021No Hit
ACCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3551.32240640715217No Hit
TCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3491.3000558763270629No Hit
TCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3491.3000558763270629No Hit
GCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3301.2292791953808901No Hit
TCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3291.225554106910039No Hit
GCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3281.221829018439188No Hit
CCCTACGGGAGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3131.1659526913764202No Hit
GCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA3051.1361519836096108No Hit
ACCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2991.1138014527845037No Hit
TCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2961.10262618737195No Hit
ACCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2911.084000745017694No Hit
ACCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2851.061650214192587No Hit
CCCTACGGGTGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2781.0355745948966288No Hit
GCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2751.0243993294840752No Hit
CCCTACGGGGCGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2721.0132240640715218No Hit
CCCTACGGGGGGCTGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2660.9908735332464147No Hit
GCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2600.9685230024213075No Hit
ACCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2530.9424473831253493No Hit
CCCTACGGGAGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2290.8530452598249207No Hit
CCCTACGGGTGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2250.8381449059415161No Hit
CCCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2220.8269696405289625No Hit
CCCTACGGGGGGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2200.8195194635872602No Hit
ACCTACGGGGTGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2150.8008940212330043No Hit
GCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2130.7934438442913019No Hit
TCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA2110.7859936673495995No Hit
ACCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1930.7189420748742782No Hit
GCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1710.6369901285155523No Hit
CCCTATGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1640.610914509219594No Hit
CCCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1510.5624883590985286No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1420.528962562860868No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1360.5066120320357609No Hit
ACCTACGGGGCGCAGCAGCTGAGAATCTTCCACAATGGACGAAAGTCTGA1280.47681132426895134No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.4730862357981002No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.34270813931830885No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.34270813931830885No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.3389830508474576No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.33525796237660643No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA850.3166325200223505No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.2980070776680946No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.28683181225554105No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.28683181225554105No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA750.2793816353138387No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA720.26820636990128516No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.264481281430434No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.2570311044887316No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.25330601601788044No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.24958092754702924No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.24958092754702924No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA630.2346805736636245No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.23095548519277334No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.22350530825107096No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.2160551313093686No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.2123300428385174No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.20487986589681506No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA550.20487986589681506No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.20487986589681506No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA550.20487986589681506No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA540.20115477742596388No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.19742968895511268No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA520.1937046004842615No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA510.18997951201341032No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.18997951201341032No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.18625442354255914No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA480.17880424660085678No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.17507915813000557No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.17135406965915442No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.17135406965915442No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.17135406965915442No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.16762898118830322No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.16762898118830322No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.16762898118830322No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.15645371577574968No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.15645371577574968No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.1527286273048985No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.1527286273048985No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.1527286273048985No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA410.1527286273048985No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.1490035388340473No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.14527845036319612No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.14155336189234494No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.14155336189234494No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA360.13410318495064258No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA360.13410318495064258No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.13410318495064258No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.1303780964797914No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA350.1303780964797914No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.1303780964797914No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.12665300800894022No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA340.12665300800894022No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA340.12665300800894022No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.12292791953808903No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA330.12292791953808903No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.12292791953808903No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.11920283106723784No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.11920283106723784No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.11920283106723784No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.11920283106723784No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA310.11547774259638667No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.11547774259638667No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.11547774259638667No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA310.11547774259638667No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.1080275656546843No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA280.10430247718383312No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA280.10430247718383312No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA280.10430247718383312No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.10057738871298194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)