FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29898
Sequences flagged as poor quality0
Sequence length321
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA19466.50879657502174No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA7802.608870158539033No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA6052.0235467255334805No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA5631.883069101612148No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA5631.883069101612148No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA5251.7559702990166566No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA5101.705799719044752No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4861.6255267910897049No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4711.5753562111178006No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4521.5118068098200548No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4461.491738577831293No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4331.4482574085223092No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4251.4214997658706268No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4211.4081209445447858No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA4141.3847080072245637No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3861.2910562579436753No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3821.277677436617834No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3781.2642986152919928No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3621.2107833299886281No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3561.1907150979998662No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3551.1873703926684058No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3521.177336276674025No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3501.1706468660111045No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3331.1137868753762794No Hit
TCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3301.1037527593818985No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3301.1037527593818985No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3171.0602715900729147No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA3101.0368586527526926No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2840.9498963141347248No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2840.9498963141347248No Hit
CCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2700.9030704394942807No Hit
ACCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2580.8629339755167571No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2480.829486922202154No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2480.829486922202154No Hit
GCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2450.8194528062077732No Hit
CCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2400.8027292795504716No Hit
CCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2390.7993845742190112No Hit
TCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2300.7692822262358686No Hit
GCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2250.7525586995785671No Hit
CCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2240.7492139942471068No Hit
ACCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2070.6923540036122818No Hit
ACCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA2050.6856645929493612No Hit
CCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1930.6455281289718376No Hit
ACCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1830.6120810756572347No Hit
GCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1830.6120810756572347No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.5585657903538699No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.4749481570673624No Hit
CCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1290.43146698775837855No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1200.4013646397752358No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1190.39801993444377554No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.3746069971235534No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.36791758646063283No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.3378152384774901No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.32109171182018864No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.31774700648872833No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.314402301157268No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.314402301157268No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.3110575958258077No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.30436818516288716No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.30102347983142685No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.29098936383704593No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.28095524784266507No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA840.28095524784266507No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.27761054251120476No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.27426583717974445No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA800.2675764265168239No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.2608870158539033No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.2608870158539033No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.257542310522443No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.24416348919660177No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.2374740785336812No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.2374740785336812No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.23412937320222088No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.23412937320222088No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA680.2274399625393003No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA670.22409525720784No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.22075055187637968No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.2174058465449194No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.19733761455615761No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.1939929092246973No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.190648203893237No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.190648203893237No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.190648203893237No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.190648203893237No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.1873034985617767No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.1873034985617767No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA550.18395879323031641No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGCCTGA530.1772693825673958No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA490.16389056124155463No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.157201150578634No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.15051173991571343No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.14716703458425312No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.14716703458425312No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.14047762392133253No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.14047762392133253No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.14047762392133253No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.13378821325841195No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.13044350792695164No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.12709880259549133No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.12709880259549133No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.12709880259549133No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.12709880259549133No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.12375409726403104No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.12375409726403104No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.12040939193257075No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.12040939193257075No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.12040939193257075No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.11706468660111044No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.11706468660111044No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.11371998126965015No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA340.11371998126965015No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.11371998126965015No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.11037527593818984No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.11037527593818984No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA320.10703057060672955No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.10703057060672955No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.10368586527526925No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.10368586527526925No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.10034115994380895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)