FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30055
Sequences flagged as poor quality0
Sequence length281
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4831.6070537348194975No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4031.3408750623856265No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3961.3175844285476626No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3801.2643486940608883No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3731.2410580602229246No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3551.1811678589253036No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3511.16785892530361No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3451.1478955248710696No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3361.1179504242222593No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3191.0613874563300616No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2970.9881883214107469No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2950.9815338545999001No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2900.9648976875727833No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.911661953086009No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2720.9050074862751621No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2350.7818998502744968No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2240.7453002828148395No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2150.7153551821660289No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.6854100815172184No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2040.6787556147063717No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1940.6454832806521377No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1920.638828813841291No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1820.6055564797870571No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.6022292463816337No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.5989020129762103No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.5922475461653636No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.57893861254367No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.5722841457328232No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.5589752121111297No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.5556479787057063No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.5523207453002829No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1640.545666278489436No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.5257028780568958No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.5223756446514723No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.5223756446514723No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.5157211778406255No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1530.5090667110297787No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.5024122442189319No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.5024122442189319No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.5024122442189319No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.4924305440026618No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.4924305440026618No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.4891033105972384No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.4891033105972384No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.4891033105972384No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.4891033105972384No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.4891033105972384No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.48244884378639163No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.48244884378639163No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.47912161038096823No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.47912161038096823No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.469139910164698No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.469139910164698No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.4658126767592747No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.4558309765430045No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.4558309765430045No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.4458492763267343No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.4458492763267343No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.4391948095158875No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.4358675761104641No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.4358675761104641No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.4325403427050407No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1290.42921310929961737No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.42255864248877056No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.41590417567792376No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.41590417567792376No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.41590417567792376No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.4125769422725004No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.4125769422725004No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.409249708867077No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.39594077524538346No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.39594077524538346No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.39261354183996006No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.39261354183996006No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.39261354183996006No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1180.39261354183996006No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.3892863084345367No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.3892863084345367No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.3793046082182665No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.3793046082182665No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.3759773748128431No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.36599567459657295No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.36599567459657295No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.36266844119114955No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.3526867409748794No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.3526867409748794No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.349359507569456No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.349359507569456No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.3427050407586092No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.32939610713691564No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.32939610713691564No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA980.32606887373149224No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.32274164032606883No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.3160871735152221No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA940.3127599401097987No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.3127599401097987No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.3094327067043753No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.3061054732989519No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA910.30277823989352853No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2927965396772584No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2927965396772584No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA860.2861420728664116No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA860.2861420728664116No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA850.2828148394609882No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.27948760605556483No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.2628514390284479No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.2595242056230245No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.2495425054067543No Hit
AGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA730.24288803859590752No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA680.22625187156879054No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA680.22625187156879054No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA600.1996340043254034No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.19297953751455665No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA520.1730161370820163No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA520.1730161370820163No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA520.1730161370820163No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA510.16968890367659292No Hit
CGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA500.16636167027116952No Hit
TGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA480.15970720346032274No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA470.15637997005489934No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA470.15637997005489934No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA460.15305273664947594No Hit
TGACTACTGGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA450.14972550324405257No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA440.1463982698386292No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA440.1463982698386292No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA430.1430710364332058No Hit
GGACTACGAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA430.1430710364332058No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA420.13974380302778242No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA420.13974380302778242No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA410.136416569622359No Hit
TGACTACGCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA400.1330893362169356No Hit
TGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA400.1330893362169356No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA390.12976210281151224No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA380.12643486940608883No Hit
CGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA380.12643486940608883No Hit
CGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA370.12310763600066545No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA370.12310763600066545No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA360.11978040259524206No Hit
GGACTACTCGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA360.11978040259524206No Hit
TGACTACTCGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA350.11645316918981867No Hit
GGAATACCGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA340.11312593578439527No Hit
AGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA340.11312593578439527No Hit
GGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA340.11312593578439527No Hit
GGACTACCAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA330.10979870237897188No Hit
TGACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA330.10979870237897188No Hit
GGACTACTGGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA320.10647146897354849No Hit
TGACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA320.10647146897354849No Hit
GGACTACAAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA320.10647146897354849No Hit
AGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA310.1031442355681251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)