FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31942
Sequences flagged as poor quality0
Sequence length281
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3621.1333041137060924No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3521.1019973702335484No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3221.0080771398159165No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3211.004946465468662No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3140.9830317450378813No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2970.9298102811345564No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2840.8891115146202493No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2760.864066119842214No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2760.864066119842214No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2690.8421513994114332No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2550.7983219585498716No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.7857992611608541No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2350.7357084716047836No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2270.7106630768267485No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2200.6887483563959677No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2010.6292655437981342No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.5823054285893182No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.5635213825057918No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.5478680107695197No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.5384759877277566No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1540.4821238494771774No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1530.478993175129923No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1520.4758625007826686No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.46960115208815983No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.463339803393651No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.44768643165737904No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.44455575731012464No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.44455575731012464No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.44142508296287025No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.432033059921107No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.42577171122659824No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.42577171122659824No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.41637968818483506No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.4101183394903262No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.39759564210130866No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.39759564210130866No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.39446496775405426No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.3882036190595454No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.3882036190595454No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.38507294471229103No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.38507294471229103No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.3662888986287646No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.3662888986287646No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1160.36315822428151023No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.3506355268924926No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.3506355268924926No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.3506355268924926No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.3443741781979838No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.3443741781979838No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.34124350385072943No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.33811282950347504No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.33811282950347504No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.33811282950347504No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.33811282950347504No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.33498215515622065No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.3318514808089662No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.3318514808089662No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.32872080646171187No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.32872080646171187No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.3193287834199487No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.3161981090726943No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.31306743472543985No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.31306743472543985No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.31306743472543985No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.31306743472543985No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.30993676037818546No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.3036754116836767No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.3036754116836767No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.3005447373364223No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.3005447373364223No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.3005447373364223No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.2974140629891679No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.2942833886419135No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.2942833886419135No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.2911527142946591No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.2817606912528959No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.2786300169056415No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.2786300169056415No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA890.2786300169056415No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2754993425583871No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2754993425583871No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2754993425583871No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2754993425583871No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.2754993425583871No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.2723686682111327No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.2723686682111327No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.2723686682111327No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.2692379938638783No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA840.26297664516936947No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.26297664516936947No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.2598459708221151No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.2567152964748607No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.2535846221276063No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA800.2504539477803519No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA800.2504539477803519No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.2473232734330975No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA780.24419259908584312No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.2410619247385887No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.2379312503913343No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA720.2254085530023167No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.22227787865506232No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.22227787865506232No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.21288585561329912No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.21288585561329912No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA670.20975518126604473No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.20349383257153592No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.20349383257153592No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.20349383257153592No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.1909711351825183No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.1909711351825183No Hit
AGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA590.1847097864880095No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA580.18157911214075512No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA550.1721870890989919No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA530.1659257404044831No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15340304301546553No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15340304301546553No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15340304301546553No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15340304301546553No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA490.15340304301546553No Hit
TGACTACTCGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA440.13774967127919355No Hit
TGACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA430.13461899693193916No Hit
CGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA430.13461899693193916No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA420.13148832258468474No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA420.13148832258468474No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA420.13148832258468474No Hit
GGACTACGAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA420.13148832258468474No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA420.13148832258468474No Hit
TGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA410.12835764823743034No Hit
CGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA410.12835764823743034No Hit
TGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA400.12522697389017595No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA400.12522697389017595No Hit
TGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA400.12522697389017595No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA400.12522697389017595No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA390.12209629954292156No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA380.11896562519566715No Hit
TGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA380.11896562519566715No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA380.11896562519566715No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA380.11896562519566715No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA370.11583495084841275No Hit
GGACTACGCGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA370.11583495084841275No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA360.11270427650115836No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA360.11270427650115836No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA360.11270427650115836No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA350.10957360215390397No Hit
TGACTACGAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA350.10957360215390397No Hit
AGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA350.10957360215390397No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA340.10644292780664956No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA340.10644292780664956No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA340.10644292780664956No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA340.10644292780664956No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.10331225345939515No Hit
AGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA330.10331225345939515No Hit
AGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGAATGA330.10331225345939515No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.10331225345939515No Hit
GGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA330.10331225345939515No Hit
TGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA330.10331225345939515No Hit
TGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA330.10331225345939515No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA320.10018157911214076No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA320.10018157911214076No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA320.10018157911214076No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA320.10018157911214076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)