FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20396
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA12766.2561286526769955No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3901.9121396352225928No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3461.6964110609923515No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3231.5836438517356344No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3141.539517552461267No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3031.4855854089037066No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2981.4610707981957247No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2751.3483035889390076No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2631.289468523239851No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2621.2845656010982545No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2531.240439301823887No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2491.2208276132575013No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2411.1816042361247303No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2401.176701313983134No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2371.1619925475583448No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2341.1472837811335557No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2281.1178662482839772No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2281.1178662482839772No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2251.103157481859188No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2191.0737399490096098No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2181.0688370268680132No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2141.049225338301628No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2141.049225338301628No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2131.0443224161600313No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1970.9658756618944891No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1940.9511668954696999No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1850.9070405961953324No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1840.902137674053736No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1800.8825259854873504No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1670.8187879976465975No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1540.7550500098058444No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1530.7501470876642479No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1500.7354383212394587No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1490.7305353990978624No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1400.6864090998234947No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1390.6815061776818984No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1240.6079623455579526No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1220.5981565012747597No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1200.588350656991567No Hit
GCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA1190.5834477348499706No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1190.5834477348499706No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1120.5491272798587958No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1100.5393214355756031No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1040.5099039027260247No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA920.451068837026868No Hit
GCCTACGGGAGGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA910.44616591488527163No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA830.4069425377525005No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.4069425377525005No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA800.3922337713277113No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA700.34320454991174737No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA630.3088840949205726No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.2941753284957835No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.2794665620709943No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.25985487350460873No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA510.250049029221416No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.250049029221416No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.24514610707981957No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.24514610707981957No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.23534026279662681No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA480.23534026279662681No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.23534026279662681No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.2304373406550304No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.2304373406550304No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.2206314963718376No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.21572857423024122No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.21572857423024122No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.20592272994704844No Hit
TCCTATGGGGCGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA410.20101980780545206No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA400.19611688566385566No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA390.19121396352225925No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.18631104138066287No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.18140811923906647No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA370.18140811923906647No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA360.1765051970974701No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA360.1765051970974701No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.17160227495587368No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.17160227495587368No Hit
TCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA340.1666993528142773No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA330.16179643067268093No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.15689350853108452No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.15689350853108452No Hit
TCCTACGGGTGGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA320.15689350853108452No Hit
ACCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA300.14708766424789174No Hit
TCCTATGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA300.14708766424789174No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA300.14708766424789174No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA300.14708766424789174No Hit
GCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA290.14218474210629536No Hit
TCCTACGGGGCGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA290.14218474210629536No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.14218474210629536No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA280.13728181996469896No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA280.13728181996469896No Hit
ACCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA280.13728181996469896No Hit
TCCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA280.13728181996469896No Hit
TCCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA280.13728181996469896No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA270.13237889782310258No Hit
ACCTACGGGGGGCAGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA270.13237889782310258No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.13237889782310258No Hit
ACCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA270.13237889782310258No Hit
TCCTACGGGAGGCAGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA270.13237889782310258No Hit
ACCTACGGGTGGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA270.13237889782310258No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.13237889782310258No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT260.12747597568150618No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA260.12747597568150618No Hit
ACCTATGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA260.12747597568150618No Hit
CCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA260.12747597568150618No Hit
GCCTATGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA260.12747597568150618No Hit
GCCTACGGGTGGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA250.12257305353990978No Hit
TCCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA250.12257305353990978No Hit
TCCTACGGGGTGCAGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA250.12257305353990978No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA250.12257305353990978No Hit
TCCTACGGGAGGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA250.12257305353990978No Hit
GCCTACGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA240.11767013139831341No Hit
GCCTATGGGGCGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA240.11767013139831341No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGCAACCCTGA240.11767013139831341No Hit
GCCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA240.11767013139831341No Hit
TCCTACGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA240.11767013139831341No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA240.11767013139831341No Hit
TCCTATGGGGCGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA230.112767209256717No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA230.112767209256717No Hit
GCCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA230.112767209256717No Hit
ACCTACGGGGCGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA230.112767209256717No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA220.10786428711512061No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA220.10786428711512061No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA220.10786428711512061No Hit
GCCTACGGGGCGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA220.10786428711512061No Hit
TCCTACGGGGGGCAGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA210.10296136497352422No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA210.10296136497352422No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA210.10296136497352422No Hit
ACCTACGGGGCGCTGCAGTCGGGAATTTTGCGCAATGGAGGAAACTCTGA210.10296136497352422No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA210.10296136497352422No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA210.10296136497352422No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA210.10296136497352422No Hit
CCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA210.10296136497352422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)