FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31071
Sequences flagged as poor quality0
Sequence length321
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA16235.223520324418267No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5741.8473818029673972No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4731.5223198480898588No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4561.4676064497441343No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4271.3742718290367224No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4241.3646165234463004No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4101.319558430690998No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3991.2841556435261177No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3971.2777187731325028No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3721.1972578932123201No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3501.1264523188825593No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3471.1167970132921372No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3451.1103601428985228No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3391.091049531717679No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3201.02989926297834No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3181.0234623925847253No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3070.9880596054198448No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2960.9526568182549645No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2940.9462199478613499No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2890.9301277718773133No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2680.8625406327443597No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA2580.8303562807762865No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2540.8174825399890574No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2540.8174825399890574No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.7820797528241769No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2400.7724244472337549No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.7659875768401403No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2310.7434585304624892No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2260.7273663544784527No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2200.7080557432976087No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.659779215345499No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1910.6147211225901966No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.5921920762125454No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1820.5857552058189308No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.5825367706221235No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.556789289047665No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.5503524186540504No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.5503524186540504No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1620.5213865018827846No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1490.4795468443242895No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.47632840912748226No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.4505809275530237No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.43770718676579445No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1350.43448875156898714No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGA1260.40552283479772133No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1250.402304399600914No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.3765569180264555No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.34759100125518977No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA960.30896977889350197No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.2960960381062727No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA920.2960960381062727No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA900.2896591677126581No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.27678542692542885No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA840.2703485565318142No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA820.2639116861381996No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.257474815744585No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.257474815744585No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.25103794535097035No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA760.24460107495735575No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA700.22529046377651185No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA680.21885359338289723No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA660.21241672298928263No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.2091982877924753No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA640.205979852595668No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA630.20276141739886067No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.20276141739886067No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.19954298220205335No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA610.19632454700524607No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.19632454700524607No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.18988767661163145No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.18988767661163145No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA580.1866692414148241No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.1866692414148241No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.1834508062180168No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.1834508062180168No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.18023237102120948No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA560.18023237102120948No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA550.17701393582440217No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA550.17701393582440217No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGA530.17057706543078754No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.16735863023398023No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA520.16735863023398023No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA520.16735863023398023No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.16735863023398023No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.16414019503717292No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.1609217598403656No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA490.1577033246435583No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA490.1577033246435583No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.1577033246435583No Hit
ACCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA480.15448488944675098No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA480.15448488944675098No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA470.15126645424994367No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.15126645424994367No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.14804801905313636No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA450.14482958385632905No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA450.14482958385632905No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA450.14482958385632905No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA440.14161114865952174No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA440.14161114865952174No Hit
TCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA440.14161114865952174No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.13839271346271442No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.1319558430690998No Hit
ACCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA400.1287374078722925No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.1287374078722925No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA390.12551897267548517No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA380.12230053747867788No Hit
CCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA370.11908210228187056No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA360.11586366708506324No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.11586366708506324No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA360.11586366708506324No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA360.11586366708506324No Hit
GCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA360.11586366708506324No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGA350.11264523188825593No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGCAAGTCTGA350.11264523188825593No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA350.11264523188825593No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.11264523188825593No Hit
TCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA350.11264523188825593No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA350.11264523188825593No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.11264523188825593No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA340.10942679669144861No Hit
ACCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA340.10942679669144861No Hit
GCCTACGGGAGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGA340.10942679669144861No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA330.10620836149464132No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.10620836149464132No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA330.10620836149464132No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.102989926297834No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA320.102989926297834No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.102989926297834No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.102989926297834No Hit
CCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA320.102989926297834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)