FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22100
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA14946.760180995475114No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5052.2850678733031673No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA4251.9230769230769231No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3991.8054298642533935No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3681.665158371040724No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3581.6199095022624435No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3471.5701357466063346No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3391.5339366515837105No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3181.4389140271493213No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3031.3710407239819005No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3021.3665158371040724No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3011.3619909502262444No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3011.3619909502262444No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2821.2760180995475112No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2781.257918552036199No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2781.257918552036199No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2651.1990950226244346No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2601.1764705882352942No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2411.0904977375565612No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2321.0497737556561086No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2301.0407239819004523No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2271.0271493212669685No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2251.0180995475113122No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2221.004524886877828No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.9728506787330317No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2140.9683257918552035No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.9547511312217195No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.9366515837104072No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.9276018099547512No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2030.918552036199095No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.8597285067873304No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1850.8371040723981901No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1790.8099547511312217No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.7782805429864253No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1540.6968325791855203No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1530.6923076923076923No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.6787330316742082No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.665158371040724No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1450.6561085972850679No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1450.6561085972850679No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.6244343891402715No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1380.6244343891402715No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1350.6108597285067874No Hit
GCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1340.6063348416289592No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1330.6018099547511312No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1270.5746606334841629No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.5475113122171945No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1130.5113122171945701No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1120.5067873303167421No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1120.5067873303167421No Hit
TCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA970.4389140271493213No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.42533936651583715No Hit
ACCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA930.4208144796380091No Hit
GCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA900.40723981900452494No Hit
GCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA880.3981900452488688No Hit
ACCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA860.3891402714932127No Hit
TCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA860.3891402714932127No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA850.38461538461538464No Hit
TCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA850.38461538461538464No Hit
GCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA820.37104072398190047No Hit
ACCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA810.36651583710407243No Hit
CCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA800.36199095022624433No Hit
GCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA790.3574660633484163No Hit
ACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA760.3438914027149321No Hit
TCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA730.33031674208144796No Hit
CCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA710.3212669683257919No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA700.3167420814479638No Hit
TCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA700.3167420814479638No Hit
GCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA680.3076923076923077No Hit
CCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA680.3076923076923077No Hit
ACCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA660.2986425339366516No Hit
GCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA660.2986425339366516No Hit
GCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA650.29411764705882354No Hit
CCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA630.2850678733031674No Hit
CCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA610.27601809954751133No Hit
CCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA600.27149321266968324No Hit
ACCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA600.27149321266968324No Hit
CCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA580.26244343891402716No Hit
ACCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA580.26244343891402716No Hit
CCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA560.25339366515837103No Hit
ACCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA560.25339366515837103No Hit
TCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA510.23076923076923078No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.22171945701357468No Hit
CCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA480.2171945701357466No Hit
ACCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA460.2081447963800905No Hit
GCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA440.1990950226244344No Hit
TCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA440.1990950226244344No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.1990950226244344No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA430.19457013574660634No Hit
GCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA400.18099547511312217No Hit
CCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA380.17194570135746606No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.16742081447963802No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.16289592760180996No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA360.16289592760180996No Hit
ACCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.16289592760180996No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA340.15384615384615385No Hit
CCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA340.15384615384615385No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.1493212669683258No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA320.14479638009049772No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA310.14027149321266968No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA300.13574660633484162No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA290.13122171945701358No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.13122171945701358No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA290.13122171945701358No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA280.12669683257918551No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA280.12669683257918551No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA270.12217194570135746No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.1176470588235294No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA250.11312217194570137No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA250.11312217194570137No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA250.11312217194570137No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA250.11312217194570137No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA240.1085972850678733No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA230.10407239819004525No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA230.10407239819004525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)