FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23077
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA9704.203319322268926No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5272.283659054469818No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA4591.9889933700220999No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA3231.399662001126663No Hit
GCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA3011.304328985570048No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2641.1439961866793777No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2581.1179962733457556No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2561.1093296355678814No Hit
GCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2541.1006629977900073No Hit
ACCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2351.0183299389002036No Hit
ACCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2311.0009966633444556No Hit
TCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2280.9879967066776444No Hit
ACCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2170.9403301988993369No Hit
GCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA2050.8883303722320925No Hit
TCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1960.8493305022316593No Hit
GCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1870.8103306322312259No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1760.7626641244529185No Hit
GCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1720.7453308488971703No Hit
CCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1720.7453308488971703No Hit
TCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1690.7323308922303593No Hit
CCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1690.7323308922303593No Hit
TCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1680.7279975733414222No Hit
CCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1670.7236642544524852No Hit
ACCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1650.714997616674611No Hit
GCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1650.714997616674611No Hit
TCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1620.7019976600078001No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.697664341118863No Hit
GCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1590.6889977033409889No Hit
ACCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1570.6803310655631148No Hit
CCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1560.6759977466741778No Hit
ACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1460.6326645577848073No Hit
TCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1430.6196646011179963No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA1400.6066646444511852No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.6066646444511852No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.5676647744507518No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.5589981366728778No Hit
GCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1270.5503314988950037No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1250.5416648611171296No Hit
CCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1240.5373315422281926No Hit
CCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1220.5286649044503186No Hit
CCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1190.5156649477835074No Hit
ACCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1180.5113316288945704No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.5113316288945704No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.4939983533388222No Hit
TCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1140.4939983533388222No Hit
CCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1130.4896650344498852No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1130.4896650344498852No Hit
GCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1120.4853317155609481No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.47666507778307404No Hit
CCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1070.46366512111626296No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.46366512111626296No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA1030.4463318455605148No Hit
ACCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1010.4376652077826408No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA980.42466525111582965No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.41599861333795557No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.41599861333795557No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA950.4116652944490185No Hit
ACCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA920.39866533778220736No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.3943320188932704No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.38133206222645927No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.3769987433375222No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.37266542444858514No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA820.35533214889283704No Hit
GCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA770.3336655544481518No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA750.32499891667027775No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.3163322788924037No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.31199896000346666No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.31199896000346666No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA710.30766564111452965No Hit
CCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA690.2989990033366555No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA690.2989990033366555No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.2946656844477185No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA660.28599904666984444No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.27733240889197036No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA610.2643324522251593No Hit
TCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA590.2556658144472852No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.2556658144472852No Hit
ACCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA570.2469991766694111No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA560.24266585778047406No Hit
CCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA560.24266585778047406No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.23833253889153702No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.23833253889153702No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA540.2339992200026No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.21666594444685183No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA500.21666594444685183No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.20799930666897778No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.20799930666897778No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.19933266889110368No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.19499935000216664No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.18633271222429257No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA410.17766607444641852No Hit
ACCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA390.16899943666854444No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.1646661177796074No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.1646661177796074No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.16033279889067037No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.16033279889067037No Hit
ACCTACGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA350.1516661611127963No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA350.1516661611127963No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA350.1516661611127963No Hit
ACCTATGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA340.14733284222385926No Hit
ACCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA340.14733284222385926No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.14299952333492222No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA330.14299952333492222No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.14299952333492222No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA320.13866620444598518No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.13866620444598518No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.13866620444598518No Hit
GCCTATGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA310.13433288555704814No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA310.13433288555704814No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA310.13433288555704814No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA310.13433288555704814No Hit
TCCTACGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA300.1299995666681111No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGA300.1299995666681111No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA300.1299995666681111No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA300.1299995666681111No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA290.12566624777917407No Hit
GCCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA290.12566624777917407No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA280.12133292889023703No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA280.12133292889023703No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA280.12133292889023703No Hit
ACCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA280.12133292889023703No Hit
GCCTACGGGTGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA270.1169996100013No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.11266629111236295No Hit
GCCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA260.11266629111236295No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA260.11266629111236295No Hit
TCCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA260.11266629111236295No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA260.11266629111236295No Hit
TCCTACGGGGTGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA250.10833297222342592No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA250.10833297222342592No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA240.10399965333448889No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA240.10399965333448889No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATAGCCTGA240.10399965333448889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)