FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37915
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA20135.309244362389555No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6521.7196360279572727No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5441.4347883423447183No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5401.424238428062772No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4891.2897270209679548No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4701.239614928128709No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4481.1815903995780035No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4321.1393907424502177No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4001.054991428194646No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3991.0523539496241594No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3851.0154292496373467No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3801.0022418567849136No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3770.9943294210734538No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3720.9811420282210208No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3640.9600421996571278No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3490.9204800210998285No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3400.8967427139654491No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.8782803639720427No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3300.8703679282605828No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA3290.8677304496900964No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3270.862455492549123No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3140.8281682711327971No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3120.8228933139918237No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.8176183568508506No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2960.780693656864038No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2920.7701437425820915No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2900.7648687854411182No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.712119214031386No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2650.6989318211789529No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2620.6910193854674931No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2610.6883819068970064No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2520.6646445997626269No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2270.5987076355004616No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2270.5987076355004616No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2250.5934326783594883No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.5696953712251088No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2020.5327706712382962No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2010.5301331926678096No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.519583278385863No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1960.5169457998153765No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.5169457998153765No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.5116708426744032No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA1890.49848344982197024No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.48529605696953715No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.4114466569959119No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA1440.3797969141500725No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.3771594355795859No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1400.3692469998681261No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1280.3375972570222867No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA1200.3164974284583938No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1150.3033100356059607No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1130.29803507846498745No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.2901226427535276No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.2848476856125544No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1070.2822102070420678No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.2822102070420678No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1060.27957272847158116No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1050.2769352499010945No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1050.2769352499010945No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA1040.27429777133060795No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA960.25319794276671503No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA930.2452855070552552No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA930.2452855070552552No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.24264802848476857No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.23737307134379534No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA900.23737307134379534No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.23473559277330872No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.23473559277330872No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGA870.22946063563233549No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA830.21891072135038903No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA830.21891072135038903No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA830.21891072135038903No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA810.2136357642094158No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA810.2136357642094158No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA770.20308584992746936No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA750.1978108927864961No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA740.1951734142160095No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA720.18989845707503625No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA720.18989845707503625No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA690.18198602136357642No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA680.1793485427930898No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA680.1793485427930898No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA650.17143610708162996No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.16352367137017013No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.16352367137017013No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.16352367137017013No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA620.16352367137017013No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA610.1608861927996835No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA610.1608861927996835No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.1608861927996835No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA600.1582487142291969No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.1582487142291969No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.1582487142291969No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA600.1582487142291969No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA590.15561123565871027No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.15561123565871027No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.15297375708822367No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA580.15297375708822367No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.15297375708822367No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.15033627851773704No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA570.15033627851773704No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA560.14769879994725044No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA560.14769879994725044No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA560.14769879994725044No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA550.1450613213767638No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA550.1450613213767638No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA540.1424238428062772No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA540.1424238428062772No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA530.13978636423579058No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA530.13978636423579058No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.13978636423579058No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.13714888566530398No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.13451140709481735No Hit
GCCTACGGGGTGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA500.13187392852433075No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA500.13187392852433075No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.13187392852433075No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA490.12923644995384412No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA490.12923644995384412No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA490.12923644995384412No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA490.12923644995384412No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA480.12659897138335752No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA480.12659897138335752No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA480.12659897138335752No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA470.12396149281287089No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA470.12396149281287089No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA470.12396149281287089No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA470.12396149281287089No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA470.12396149281287089No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.12396149281287089No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA460.12132401424238429No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA460.12132401424238429No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA450.11868653567189767No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA440.11604905710141104No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.11604905710141104No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA440.11604905710141104No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA430.11341157853092443No Hit
TCCTACGGGTGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA420.11077409996043783No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA420.11077409996043783No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA420.11077409996043783No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.11077409996043783No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA410.10813662138995121No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA410.10813662138995121No Hit
ACCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA400.10549914281946458No Hit
GCCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA400.10549914281946458No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA400.10549914281946458No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA390.10286166424897797No Hit
ACCTACGGGGTGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA390.10286166424897797No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA390.10286166424897797No Hit
TCCTACGGGGCGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA390.10286166424897797No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA380.10022418567849137No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGA380.10022418567849137No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA380.10022418567849137No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA380.10022418567849137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)