FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32461
Sequences flagged as poor quality0
Sequence length321
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA18175.597486214226302No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA5391.6604540833615722No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA5381.6573734635408646No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5311.6358091247959088No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4401.3554727211114876No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4221.300021564338745No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4071.253812267028126No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4061.2507316472074181No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3831.179877391331136No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3601.1090231354548536No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3591.1059425156341456No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3441.0597332183235266No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3351.0320076399371554No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3311.0196851606543238No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3230.9950402020886602No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3200.9857983426265364No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.9549921444194571No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2960.9118634669295461No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2940.9057022272881304No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2940.9057022272881304No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2870.8841378885431749No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2840.8748960290810511No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2770.8533316903360957No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.8317673515911401No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2650.8163642524876005No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2600.8009611533840608No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.7855580542805213No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2500.7701549551769816No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2480.7639937155355657No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2350.7239456578663628No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.7085425587628231No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2180.671575120914328No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.6376883028865408No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.6346076830658328No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.5914790055759219No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1910.588398385755214No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.5822371461137981No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1860.5729952866516743No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1770.545269708265303No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.5360278488031791No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.5329472289824713No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.5175441298789316No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.4805766920304365No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.4682542127476048No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1520.4682542127476048No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.45593173346477306No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.4528511136440652No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.4405286343612335No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1420.43744801454052556No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1390.42820615507840176No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1370.4220449154369859No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1340.4128030559748621No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1330.4097224361541542No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1330.4097224361541542No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1300.4004805766920304No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1230.37891623794707496No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1210.3727549983056591No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1160.35735189920211946No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1150.35427127938141156No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1150.35427127938141156No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1140.3511906595607036No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1130.34811003973999566No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1120.34502941991928776No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1120.34502941991928776No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1120.34502941991928776No Hit
TCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1120.34502941991928776No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1110.3419488000985798No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1090.335787560457164No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1060.3265457009950402No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1050.32346508117433226No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1010.3111426018915006No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA990.3049813622500847No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA990.3049813622500847No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA990.3049813622500847No Hit
CCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA990.3049813622500847No Hit
ACCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA980.3019007424293768No Hit
GCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA980.3019007424293768No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA970.29882012260866886No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA960.29573950278796096No Hit
GCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA960.29573950278796096No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA960.29573950278796096No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA950.292658882967253No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA940.28957826314654506No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA940.28957826314654506No Hit
TCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA940.28957826314654506No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA930.28649764332583716No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA920.2834170235051292No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA920.2834170235051292No Hit
CCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA920.2834170235051292No Hit
GCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA910.2803364036844213No Hit
ACCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.2772557838637134No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA890.27417516404300546No Hit
CCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA890.27417516404300546No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA880.27109454422229756No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA880.27109454422229756No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA870.26801392440158955No Hit
GCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA830.2556914451187579No Hit
ACCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA830.2556914451187579No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA810.24953020547734203No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA810.24953020547734203No Hit
ACCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA800.2464495856566341No Hit
ACCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA790.24336896583592618No Hit
TCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA770.23720772619451033No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA760.2341271063738024No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA760.2341271063738024No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA740.22796586673238653No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA720.2218046270909707No Hit
TCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA720.2218046270909707No Hit
CCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA680.20948214780813898No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA680.20948214780813898No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA670.20640152798743105No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA670.20640152798743105No Hit
CCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA650.2002402883460152No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA650.2002402883460152No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA630.19407904870459936No Hit
CCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA630.19407904870459936No Hit
GCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA610.1879178090631835No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.1879178090631835No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.1879178090631835No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA590.18175656942176766No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA570.1755953297803518No Hit
CCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA560.17251470995964388No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA560.17251470995964388No Hit
CCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA550.16943409013893596No Hit
ACCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA550.16943409013893596No Hit
ACCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA540.16635347031822803No Hit
GCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA530.1632728504975201No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.16019223067681218No Hit
CCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA520.16019223067681218No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.15711161085610426No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.15711161085610426No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.1540309910353963No Hit
ACCTACGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA500.1540309910353963No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.1509503712146884No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.1509503712146884No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA470.14478913157327253No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.14478913157327253No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.1386278919318567No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.13554727211114878No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.13554727211114878No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.13246665229044083No Hit
TCCTACGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA430.13246665229044083No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.13246665229044083No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.1293860324697329No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.1293860324697329No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.1293860324697329No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.1293860324697329No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.12630541264902498No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA400.12322479282831705No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.12322479282831705No Hit
GCCTACGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.12014417300760913No Hit
CCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.12014417300760913No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.11398293336619326No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.11090231354548535No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA360.11090231354548535No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.11090231354548535No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.10782169372477743No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.10782169372477743No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.10782169372477743No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.10782169372477743No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.10474107390406949No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.10474107390406949No Hit
CCCTACGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA330.10166045408336156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)