FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44042
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA18814.270923209663503No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA14953.394487080514055No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5671.287407474683257No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4741.0762454021161618No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4481.0172108441941783No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4390.9967758049134917No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4180.9490940465918896No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4170.9468234866718132No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3980.9036828481903637No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3870.8787066890695244No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3710.842377730348304No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3660.8310249307479225No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3570.8105898914672359No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3480.7901548521865491No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3460.7856137323463966No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA3410.7742609327460152No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3380.7674492529857863No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3370.76517869306571No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.7560964533854049No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.7560964533854049No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3300.749284773625176No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3280.7447436537850234No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3220.7311202942645656No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3190.7243086145043368No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3180.7220380545842605No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3100.7038735752236501No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3030.6879796557831162No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3010.6834385359429636No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3000.6811679760228873No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA2960.6720857363425821No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2950.6698151764225058No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2950.6698151764225058No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2930.6652740565823532No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2930.6652740565823532No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2890.6561918169020481No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2870.6516506970618955No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2870.6516506970618955No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2760.6266745379410563No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2740.6221334181009037No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2710.6153217383406748No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2690.6107806185005222No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2680.6085100585804459No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2680.6085100585804459No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2590.5880750192997594No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2560.5812633395395305No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2560.5812633395395305No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2520.5721810998592253No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.5608283002588439No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2380.5403932609781572No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.5290404613777757No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2320.5267699014576995No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2290.5199582216974706No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2270.515417101857318No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA2260.5131465419372417No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2200.499523182416784No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2190.49725262249670765No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2180.49498206257663147No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2160.49044094273647876No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2160.49044094273647876No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.48362926297625No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.4790881431360973No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2100.4768175832160211No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2050.46546478361563964No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2020.45865310385541075No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA2020.45865310385541075No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1990.45184142409518185No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1980.4495708641751056No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.4473003042550293No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.4382180645747242No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.4291358248944189No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1850.4200535852141138No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1840.4177830252940375No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1730.3928068661731983No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.37918350665274053No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1660.3769129467326643No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1590.36101902729213026No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.35647790745197766No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1550.35193678761182506No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1470.3337723082512148No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1470.3337723082512148No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1420.3224195086508333No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1400.3178783888106807No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1330.30198446937014667No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1290.2929022296898415No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.2929022296898415No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA1230.27927887016938374No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.2747377503292312No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1140.2588438308886971No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.24522047136823938No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.24522047136823938No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1000.22705599200762908No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA990.2247854320875528No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.21343263248717134No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA930.21116207256709507No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA910.20662095272694245No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA900.20435039280686618No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA860.195268153126561No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA810.18391535352617958No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA810.18391535352617958No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA800.18164479360610325No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.18164479360610325No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA790.17937423368602698No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA790.17937423368602698No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA750.17029199400572181No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA740.16802143408564552No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA730.16575087416556925No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA710.16120975432541665No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA700.15893919440534035No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA670.15212751464511148No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA670.15212751464511148No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA670.15212751464511148No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA660.14985695472503519No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA660.14985695472503519No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA660.14985695472503519No Hit
TCCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA630.14304527496480632No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA620.14077471504473002No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA620.14077471504473002No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.13850415512465375No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGTCTGA610.13850415512465375No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA610.13850415512465375No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA590.13396303528450115No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA570.12942191544434856No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA560.12715135552427229No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA560.12715135552427229No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA560.12715135552427229No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA550.124880795604196No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA550.124880795604196No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA540.12261023568411969No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA540.12261023568411969No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA530.12033967576404342No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA530.12033967576404342No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA520.11806911584396713No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA520.11806911584396713No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA510.11579855592389082No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA510.11579855592389082No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.11352799600381454No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.11352799600381454No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.11352799600381454No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA490.11125743608373824No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA490.11125743608373824No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA490.11125743608373824No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA490.11125743608373824No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA490.11125743608373824No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA480.10898687616366196No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA480.10898687616366196No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA480.10898687616366196No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA480.10898687616366196No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA480.10898687616366196No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA470.10671631624358567No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA470.10671631624358567No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA470.10671631624358567No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA460.10444575632350937No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA450.10217519640343309No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA450.10217519640343309No Hit
CCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA450.10217519640343309No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA450.10217519640343309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)