FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60405
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG40016.623623872196011No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG15132.5047595397731977No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG10511.739922191871534No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG10391.7200562867312308No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG10261.6985348894959027No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG9841.6290042215048424No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG9711.607482824269514No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG8931.4783544408575449No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG8651.432000662196838No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG8391.3889578677261816No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG8001.324393676020197No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG7441.231686118698783No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG7361.2184421819385811No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG7311.210164721463455No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6901.1422895455674198No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6891.1406340534723947No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6891.1406340534723947No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6831.1307011009022432No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6681.1058687194768646No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6591.0909692906216373No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6571.0876583064315868No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6511.0777253538614353No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6481.0727588775763595No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6461.0694478933863092No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6441.0661369091962585No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6281.0396490356758545No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6221.0297160831057033No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG6000.9932952570151479No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG5780.9568744309245922No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG5750.9519079546395167No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG5210.8625113815081533No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG5160.854233921033027No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG5160.854233921033027No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4690.7764257925668405No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4640.7681483320917143No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4510.746626934856386No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4440.7350384901912094No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA4440.7350384901912094No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4300.7118616008608558No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG4190.6936511878155782No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG3860.6390199486797451No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG3660.6059101067792401No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3470.5744557569737604No Hit
CCTGCGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3420.5661782964986343No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG3370.5579008360235079No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3280.5430014071682807No Hit
CCTATGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3220.5330684545981292No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3210.5314129625031041No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG3060.5065805810777253No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2740.4536048340369175No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2490.41221753166128633No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2100.3476533399553017No Hit
CCTACGGGAGACAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2100.3476533399553017No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1860.30792152967469577No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1770.2930221008194686No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1680.2781226719642414No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1610.2665342272990646No Hit
CCTGCGGGAGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1580.2615677510139889No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1570.25991225891896363No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1420.23507987749358497No Hit
TCTACGGAAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1400.23176889330353448No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1400.23176889330353448No Hit
CCTATGGGAGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1330.22018044863835773No Hit
CCTGCGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1200.19865905140302956No Hit
CCTACGGGGGCCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1160.19203708302292857No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1130.18707060673785283No Hit
TCTACGGTTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1120.1854151146428276No Hit
CCTACGGGAGACAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT1100.1821041304527771No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1100.1821041304527771No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1070.17713765416770133No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1050.17382666997765084No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1040.17217117788262562No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1030.17051568578760037No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1020.16886019369257513No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA1000.16554920950252464No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA990.1638937174074994No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA980.16223822531247414No Hit
CCTATGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT970.1605827332174489No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT960.15892724112242365No Hit
CCTACGGGAGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT940.15561625693237313No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA920.15230527274232264No Hit
CCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA910.1506497806472974No Hit
CCTACGGGAGCCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA900.14899428855227217No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA900.14899428855227217No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA890.14733879645724693No Hit
CCTACGGGGTGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT880.14568330436222168No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.14568330436222168No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA880.14568330436222168No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.14402781226719644No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA860.1423723201721712No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA860.1423723201721712No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA850.14071682807714594No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA830.13740584388709542No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA830.13740584388709542No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA820.13575035179207018No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA810.13409485969704493No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA800.1324393676020197No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT800.1324393676020197No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.1291283834119692No Hit
CCTGCGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA750.12416190712689348No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA740.12250641503186822No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA740.12250641503186822No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA730.12085092293684298No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.11919543084181773No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA720.11919543084181773No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT720.11919543084181773No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA710.11753993874679249No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA700.11588444665176724No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.11257346246171675No Hit
CCTACGGGAGACAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT650.107606986176641No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA650.107606986176641No Hit
TCTACGGAAGGCAGCAGTAGGGAATATTGCACAATGGAGGAAACTCTGAT640.10595149408161575No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA630.1042960019865905No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA630.1042960019865905No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA620.10264050989156528No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.10264050989156528No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA610.10098501779654002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)