FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25068
Sequences flagged as poor quality0
Sequence length281
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5272.1022817935216214No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5052.0145205042284986No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4691.870911121748843No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4681.8669219722355195No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4581.827030477102282No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4321.723312589755864No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4231.6874102441359502No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4181.6674644965693315No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3981.5876815063028564No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3651.4560395723631723No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3361.340354236476783No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3261.3004627413435454No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3101.2366363491303654No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3071.2246689005903941No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3021.2047231530237754No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2901.1568533588638903No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2681.0690920695707675No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2611.0411680229775013No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2611.0411680229775013No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.8656454443912559No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2160.861656294877932No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.8536779958512845No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2090.8337322482846657No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2080.8297430987713419No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2070.8257539492580183No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.8217647997446944No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.8217647997446944No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2060.8217647997446944No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2050.8177756502313708No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1980.7898516036381044No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1950.777884155098133No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1910.761927557044838No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1890.7539492580181906No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1870.745970958991543No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.7340035104515718No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.7340035104515718No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.7180469123982767No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.7180469123982767No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.7140577628849529No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1790.7140577628849529No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.7100686133716292No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1740.6941120153183341No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.6901228658050104No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.6861337162916866No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.6661879687250678No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1640.6542205201850966No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1640.6542205201850966No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1630.6502313706717727No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.6382639221318015No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1590.6342747726184778No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.630285623105154No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.630285623105154No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.6183181745651827No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.6183181745651827No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1540.6143290250518589No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.6023615765118877No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.5943832774852401No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.5903941279719164No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.5903941279719164No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.5824158289452689No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1460.5824158289452689No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.5704483804052976No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.5704483804052976No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.5704483804052976No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1410.5624700813786501No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.5584809318653263No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.5544917823520026No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.5544917823520026No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.5505026328386788No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.5505026328386788No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.546513483325355No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.5385351842987075No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.5345460347853838No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.5345460347853838No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.5345460347853838No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.53055688527206No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.5185894367320887No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.5106111377054412No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.5106111377054412No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.5106111377054412No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.5026328386787937No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.4986436891654699No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.4946545396521462No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.47869794159885115No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.46673049305887987No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1160.46274134354555607No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1160.46274134354555607No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.45875219403223233No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.45875219403223233No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.4547630445189086No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.4547630445189086No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.44678474549226105No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.4427955959789373No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.43481729695228977No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.4228498484123185No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.41886069889899474No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.41886069889899474No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.40689325035902346No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.3949258018190522No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA970.38694750279240464No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.37498005425243336No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.37498005425243336No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.3670017552257859No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.34705600765916705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)