FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39832
Sequences flagged as poor quality0
Sequence length321
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA18274.586764410524202No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA8512.1364731873870255No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5851.468668407310705No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA5581.4008837115886723No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4921.2351877887125928No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4901.2301667001405905No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4211.0569391444065075No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4081.0243020686884918No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4071.0217915244024904No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4031.0117493472584858No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3980.9991966258284796No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3950.9916649929704761No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3720.9339224743924482No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3630.9113275758184375No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3320.8335007029524002No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3190.8008636272343845No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3190.8008636272343845No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3150.7908214500903796No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3140.7883109058043785No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3060.7682265515163688No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3050.7657160072303676No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2910.7305683872263506No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.723036754368347No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2830.7104840329383411No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2770.6954207672223338No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2720.6828680457923277No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.6778469572203254No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.6703153243623218No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.6602731472183169No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2490.6251255272143No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.5949989957822855No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2170.5447881100622615No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2140.5372564772042578No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2100.527214300060253No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2070.5196826672022494No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.5171721229162483No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2050.5146615786302471No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2040.5121510343442459No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2030.5096404900582446No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2030.5096404900582446No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.4995983129142398No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1970.4945772243422374No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1970.4945772243422374No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.47700341434022897No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1880.4719823257682266No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1870.46947178148222535No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA1850.46445069291022295No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1830.45942960433822055No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1830.45942960433822055No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.45189797148021693No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1800.45189797148021693No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.4343241614782085No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.41926089576220127No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1670.41926089576220127No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1610.404197630046194No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1600.40168708576019285No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.39164490861618795No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1540.3866238200441856No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1540.3866238200441856No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1490.37407109861417953No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1490.37407109861417953No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1480.37156055432817836No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1480.37156055432817836No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.36905001004217713No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1450.36402892147017474No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1440.3615183771841735No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1410.3539867443261699No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1380.34645511146816627No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1380.34645511146816627No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1370.3439445671821651No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1340.33641293432416147No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1340.33641293432416147No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1330.33390239003816025No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1320.3313918457521591No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1310.32888130146615785No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1280.3213496686081542No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1270.31883912432215306No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1220.306286402892147No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1210.3037758586061458No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1180.2962442257481422No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.2962442257481422No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1170.29373368146214096No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1170.29373368146214096No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA1150.28871259289013856No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1150.28871259289013856No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.28118096003213494No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1120.28118096003213494No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1120.28118096003213494No Hit
GCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1080.27113878288813015No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.27113878288813015No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1040.26109660574412535No Hit
GCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1040.26109660574412535No Hit
GCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1030.25858606145812413No Hit
ACCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1030.25858606145812413No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1020.25607551717212296No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1020.25607551717212296No Hit
TCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1000.2510544286001205No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA980.24603334002811808No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA970.24352279574211688No Hit
CCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA960.24101225145611568No Hit
CCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA950.23850170717011449No Hit
ACCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA910.22845953002610966No Hit
ACCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA910.22845953002610966No Hit
TCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.22594898574010847No Hit
GCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.22594898574010847No Hit
CCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA900.22594898574010847No Hit
ACCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA890.22343844145410724No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.22092789716810607No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.21590680859610364No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.21590680859610364No Hit
TCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA860.21590680859610364No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA850.21339626431010245No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA850.21339626431010245No Hit
ACCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA790.1983329985940952No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA790.1983329985940952No Hit
CCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA790.1983329985940952No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.19582245430809397No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA780.19582245430809397No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA780.19582245430809397No Hit
GCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA750.18829082145009038No Hit
TCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA750.18829082145009038No Hit
TCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA740.18578027716408918No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.18075918859208676No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA710.17824864430608556No Hit
CCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA700.17573810002008436No Hit
CCCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA690.17322755573408313No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA640.1606748343040771No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.15816429001807591No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.15816429001807591No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.15565374573207472No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.1506326571600723No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGTCTGA590.1481221128740711No Hit
CCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA590.1481221128740711No Hit
ACCTACGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA580.1456115685880699No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGGAACCCTGA580.1456115685880699No Hit
GCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA560.14059048001606747No Hit
CCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA550.13807993573006627No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA550.13807993573006627No Hit
ACCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA540.13556939144406507No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA530.13305884715806388No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.13305884715806388No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA510.12803775858606148No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA510.12803775858606148No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.12552721430006025No Hit
CCCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA490.12301667001405904No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA480.12050612572805784No Hit
ACCTATGGGGCGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA480.12050612572805784No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGA480.12050612572805784No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.11548503715605543No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA440.11046394858405303No Hit
CCCTACGGGGTGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA440.11046394858405303No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.11046394858405303No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA430.10795340429805182No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.10795340429805182No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA430.10795340429805182No Hit
ACCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.10544286001205061No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.10544286001205061No Hit
GCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.10544286001205061No Hit
TCCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.10544286001205061No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.10544286001205061No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA410.10293231572604941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)