FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34747
Sequences flagged as poor quality0
Sequence length321
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA16054.619103807522952No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5051.4533628802486547No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA4261.2260051227444095No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4171.2001036060667107No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3791.0907416467608715No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3741.076351915273261No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3681.0590842374881286No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3521.013037096727775No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3501.0072812041327308No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3320.9554781707773333No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.8864074596368031No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.8864074596368031No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2900.8346044262814055No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2860.8230926410913173No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA2800.8058249633061848No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2770.7971911244136185No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2730.7856793392235301No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2620.7540219299507872No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2490.716608628083No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2480.7137306817854779No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.7108527354879558No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2300.6619276484300803No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2220.6389040780499036No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2180.6273922928598152No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.6187584539672489No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2140.6158805076697269No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.6072466687771606No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2070.5957348835870723No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2060.5928569372895501No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2030.5842230983969839No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.5583215817192851No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1860.5352980113391084No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1860.5352980113391084No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.520908279851498No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1760.5065185483638875No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.5036406020663654No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.4633493539010562No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.423058105735747No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.4144242668431807No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.4144242668431807No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.41154632054565865No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1410.40579042795061443No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.39427864276052615No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGA1370.39427864276052615No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1320.37988891127291563No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.3770109649753936No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.3712550723803494No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1230.35398739459521683No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1170.33671971681008434No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1140.3280858779175181No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1130.32520793161999595No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.3194520390249518No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1030.29642846864477507No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1000.2877946297522088No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.27628284456212043No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA950.2734048982645984No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA950.2734048982645984No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA910.26189311307451No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA910.26189311307451No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA880.25325927418194377No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA880.25325927418194377No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA880.25325927418194377No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA840.24174748899185539No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA830.23886954269433333No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.23886954269433333No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA830.23886954269433333No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA820.23599159639681125No Hit
ACCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA810.23311365009928914No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA770.22160186490920078No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA760.2187239186116787No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA760.2187239186116787No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA750.21584597231415664No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA740.2129680260166345No Hit
GCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA740.2129680260166345No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA740.2129680260166345No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA740.2129680260166345No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA730.21009007971911245No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA730.21009007971911245No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA720.20721213342159034No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA710.20433418712406826No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA700.2014562408265462No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA700.2014562408265462No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA660.18994445563645782No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA650.18706650933893573No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA640.18418856304141365No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA640.18418856304141365No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA620.17843267044636948No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.17555472414884737No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.17555472414884737No Hit
CCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA600.1726767778513253No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.1726767778513253No Hit
TCCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA590.1697988315538032No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA580.1669208852562811No Hit
GCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA580.1669208852562811No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA570.16404293895875904No Hit
GCCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA550.15828704636371485No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA550.15828704636371485No Hit
ACCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA550.15828704636371485No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA540.15540910006619277No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA540.15540910006619277No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA540.15540910006619277No Hit
ACCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA520.1496532074711486No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.1496532074711486No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.1496532074711486No Hit
TCCTACGGGGTGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA500.1438973148761044No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA500.1438973148761044No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA500.1438973148761044No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.14101936857858233No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.14101936857858233No Hit
GCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA480.13814142228106022No Hit
GCCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA480.13814142228106022No Hit
CCCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA470.13526347598353813No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA470.13526347598353813No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA470.13526347598353813No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.13526347598353813No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA470.13526347598353813No Hit
CCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA470.13526347598353813No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.13238552968601608No Hit
GCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA460.13238552968601608No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.13238552968601608No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.12662963709097189No Hit
ACCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA440.12662963709097189No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA440.12662963709097189No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGA430.12375169079344979No Hit
TCCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA430.12375169079344979No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA430.12375169079344979No Hit
TCCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA430.12375169079344979No Hit
TCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.12087374449592769No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA420.12087374449592769No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA420.12087374449592769No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA420.12087374449592769No Hit
GCCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.12087374449592769No Hit
ACCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA420.12087374449592769No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.12087374449592769No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA410.11799579819840562No Hit
GCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA400.11511785190088354No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.11511785190088354No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA400.11511785190088354No Hit
ACCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA390.11223990560336145No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGA390.11223990560336145No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.11223990560336145No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA390.11223990560336145No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA390.11223990560336145No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA380.10936195930583935No Hit
GCCTATGGGGCGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA380.10936195930583935No Hit
TCCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA380.10936195930583935No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA380.10936195930583935No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.10648401300831725No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.10648401300831725No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA370.10648401300831725No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA370.10648401300831725No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA370.10648401300831725No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA360.10360606671079517No Hit
TCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.10360606671079517No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.10360606671079517No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.10360606671079517No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA360.10360606671079517No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.10360606671079517No Hit
GCCTACGGGAGGCTGCAGTGAGGGATATTGGGCAATGGGGGAAACCCTGA360.10360606671079517No Hit
GCCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA360.10360606671079517No Hit
CCCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA360.10360606671079517No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA350.1007281204132731No Hit
TCCTATGGGGCGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA350.1007281204132731No Hit
GCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA350.1007281204132731No Hit
TCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA350.1007281204132731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)