FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19716
Sequences flagged as poor quality0
Sequence length321
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA10155.148103063501725No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3962.008520998174072No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2881.4607425441265978No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2751.3948062487319943No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2711.3745181578413472No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2521.278149726110773No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2451.2426455670521404No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2351.1919253398255223No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2191.1107729762629337No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2171.10062893081761No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2001.0144045445323595No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1850.9383242036924324No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.9129640900791236No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.9028200446338No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.8876039764658147No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1730.8774599310204909No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.8673158855751673No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.8571718401298438No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1680.852099817407182No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.8470277946845202No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.8165956583485494No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.7912355447352405No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.7709474538445932No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.7658754311219315No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.7658754311219315No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1510.7658754311219315No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.7405153175086224No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.725299249340637No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.6948671130046663No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.6796510448366808No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.6644349766686956No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA1270.6441468857780482No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.6441468857780482No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.623858794887401No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1100.5579224994927977No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1090.5528504767701359No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1050.5325623858794888No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.527490363156827No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1040.527490363156827No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1040.527490363156827No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.4716981132075472No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.4666260904848854No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA870.4412659768715764No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.4412659768715764No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA730.3702576587543112No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA720.36518563603164944No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.3398255224183404No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA580.29417731791438423No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGA560.2840332724690607No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.27896124974639885No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA500.2536011361330899No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA490.24852911341042808No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.2383850679651045No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA420.2130249543517955No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.2130249543517955No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA420.2130249543517955No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.2130249543517955No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA380.1927368634611483No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.1927368634611483No Hit
TCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA370.1876648407384865No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA370.1876648407384865No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.17752079529316292No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.17752079529316292No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA350.17752079529316292No Hit
TCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA350.17752079529316292No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA330.16737674984783932No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.16737674984783932No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.16737674984783932No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA320.16230472712517752No Hit
TCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA320.16230472712517752No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA320.16230472712517752No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGA320.16230472712517752No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.16230472712517752No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.15723270440251574No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA310.15723270440251574No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.15723270440251574No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA300.15216068167985392No Hit
TCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA300.15216068167985392No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA290.14708865895719211No Hit
GCCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA280.14201663623453034No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.14201663623453034No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA280.14201663623453034No Hit
GCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA280.14201663623453034No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.14201663623453034No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.14201663623453034No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.14201663623453034No Hit
ACCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA280.14201663623453034No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA270.13694461351186854No Hit
GCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA270.13694461351186854No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.13694461351186854No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.13694461351186854No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA270.13694461351186854No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.13694461351186854No Hit
ACCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA270.13694461351186854No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA270.13694461351186854No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA270.13694461351186854No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.13187259078920674No Hit
CCCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA260.13187259078920674No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGA260.13187259078920674No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA260.13187259078920674No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.13187259078920674No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA260.13187259078920674No Hit
GCCTACGGGAGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGA260.13187259078920674No Hit
TCCTACGGGGTGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA250.12680056806654494No Hit
TCCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA250.12680056806654494No Hit
GCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA250.12680056806654494No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA250.12680056806654494No Hit
GCCTACGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA250.12680056806654494No Hit
CCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA240.12172854534388314No Hit
ACCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA240.12172854534388314No Hit
ACCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA230.11665652262122135No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11665652262122135No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11665652262122135No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGA230.11665652262122135No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA230.11665652262122135No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA220.11158449989855954No Hit
GCCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA220.11158449989855954No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA220.11158449989855954No Hit
TCCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA220.11158449989855954No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA220.11158449989855954No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA220.11158449989855954No Hit
ACCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA220.11158449989855954No Hit
ACCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA220.11158449989855954No Hit
CCCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA210.10651247717589775No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT210.10651247717589775No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA200.10144045445323595No Hit
CCCTATGGGGCGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA200.10144045445323595No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10144045445323595No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10144045445323595No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA200.10144045445323595No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA200.10144045445323595No Hit
GCCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA200.10144045445323595No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA200.10144045445323595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)