FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29297
Sequences flagged as poor quality0
Sequence length281
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4091.3960473768645254No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3661.249274669761409No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3441.1741816568249308No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3411.1639417005154111No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3311.129808512817012No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3241.1059152814281326No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3021.0308222684916544No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2991.0205823121821347No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2730.9318360241662969No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.839676417380619No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2440.8328497798409393No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2410.8226098235314196No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2250.7679967232139809No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2230.7611700856743011No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2220.7577567669044611No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2160.7372768542854218No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1920.6553572038092638No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.6348772911902243No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.5802641908727856No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.5734375533331058No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.5700242345632658No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1660.5666109157934259No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.5358910468648668No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.5324777280950268No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1540.525651090555347No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1500.5119978154759873No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.5017578591664675No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.4915179028569478No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.4915179028569478No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.4915179028569478No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.484691265317268No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.47786462777758815No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1400.47786462777758815No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.46762467146806835No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1330.4539713963887087No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.45055807761886885No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.45055807761886885No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.44714475884902893No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.44373144007918897No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.44373144007918897No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.4369048025395092No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.4300781649998293No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.4266648462299894No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1250.4266648462299894No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.41642488992046967No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.41642488992046967No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.41301157115062975No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.40618493361094987No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.38911833976175036No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.38911833976175036No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.38570502099191045No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.38229170222207054No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.38229170222207054No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.37887838345223057No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.3686384271427109No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3652251083728709No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3652251083728709No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1070.3652251083728709No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1040.35498515206335124No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.3481585145236714No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.3481585145236714No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.3481585145236714No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.34474519575383145No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1010.34474519575383145No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.34133187698399153No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.34133187698399153No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.3379185582141516No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.3379185582141516No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.32085196436495206No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.32085196436495206No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.31743864559511215No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA900.3071986892855924No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.3003720517459125No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA870.2969587329760726No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.2969587329760726No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.2935454142062327No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.2935454142062327No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA860.2935454142062327No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA850.2901320954363928No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.2901320954363928No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.2867187766665529No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.2867187766665529No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.2867187766665529No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.28330545789671296No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.27647882035703314No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.27647882035703314No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.27647882035703314No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.27647882035703314No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.2696521828173533No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.2696521828173533No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.2628255452776735No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.2594122265078336No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.2594122265078336No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.25599890773799366No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.25599890773799366No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.25599890773799366No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.25258558896815375No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.25258558896815375No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.242345632658634No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.242345632658634No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA710.242345632658634No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA700.23893231388879407No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA700.23893231388879407No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.2355189951189542No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA690.2355189951189542No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA690.2355189951189542No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.23210567634911425No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.22527903880943442No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA650.22186572003959448No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.21503908249991466No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA600.20479912619039495No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA570.19455916988087518No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA550.18773253234119536No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.18773253234119536No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA530.18090589480151553No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.18090589480151553No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA500.17066593849199577No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA490.16725261972215585No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA460.15701266341263612No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA450.1535993446427962No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA430.14677270710311635No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA390.1331194320237567No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA390.1331194320237567No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA380.1297061132539168No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA360.12287947571423695No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA360.12287947571423695No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA360.12287947571423695No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA340.11605283817455712No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA340.11605283817455712No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA340.11605283817455712No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA340.11605283817455712No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.11263951940471721No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.11263951940471721No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.11263951940471721No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA330.11263951940471721No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA320.1092262006348773No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA310.10581288186503739No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA310.10581288186503739No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA310.10581288186503739No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA310.10581288186503739No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA300.10239956309519747No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA300.10239956309519747No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA300.10239956309519747No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA300.10239956309519747No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA300.10239956309519747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)