FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31135
Sequences flagged as poor quality0
Sequence length321
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA12994.172153524971897No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9903.1797013007868955No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA4181.3425405492211337No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA3401.0920186285530753No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA3371.0823831700658422No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA3191.0245704191424443No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA3181.0213585996466998No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA3171.0181467801509554No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA3110.9988758631764895No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3050.9796049462020234No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2960.9506985707403245No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2950.94748675124458No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2730.8768267223382045No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2690.8639794443552272No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2560.8222257909105508No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2450.7868957764573631No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2430.7804721374658744No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2430.7804721374658744No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2420.7772603179701301No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.7740484984743857No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.7644130399871527No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2370.7612012204914084No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2350.7547775814999197No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2310.7419303035169423No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2280.7322948450297093No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2260.7258712060382206No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2250.7226593865424763No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2180.7001766500722659No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2070.6648466356190782No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2070.6648466356190782No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA2050.6584229966275895No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2040.6552111771318452No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2030.6519993576361008No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.6391520796531235No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.6359402601573791No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1950.6263048016701461No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1940.6230929821744018No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1920.6166693431829131No Hit
TCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1880.6038220651999358No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1850.5941866067127027No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.5620684117552593No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.558856592259515No Hit
CCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1710.549221133772282No Hit
CCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1710.549221133772282No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.5460093142765376No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.5460093142765376No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.5460093142765376No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1680.539585675285049No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1660.5331620362935603No Hit
CCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1650.529950216797816No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1630.5235265778063273No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1600.5138911193190943No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1590.5106792998233499No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.5042556608318612No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1570.5042556608318612No Hit
GCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1550.4978320218403725No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1520.4881965633531395No Hit
ACCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1510.4849847438573952No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1490.47856110486590653No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1490.47856110486590653No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.47534928537016224No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.47534928537016224No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.4689256463786735No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.4689256463786735No Hit
ACCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1440.46250200738718483No Hit
GCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1380.4432310904127188No Hit
CCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1380.4432310904127188No Hit
ACCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1370.44001927091697446No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.44001927091697446No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.43038381242974144No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1230.39505379797655377No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.38863015898506503No Hit
TCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1200.3854183394893207No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.378994700497832No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1170.3757828810020877No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.3468765055403886No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.34045286654889995No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.34045286654889995No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1060.34045286654889995No Hit
GCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1050.3372410470531556No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.33081740806166693No Hit
ACCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1000.3211819495744339No Hit
CCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1000.3211819495744339No Hit
CCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA960.30833467159145656No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA920.2954873936084792No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.29227557411273486No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.29227557411273486No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA880.28264011562550184No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.2794282961297575No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.25052192066805845No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA770.2473101011723141No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA730.23446282318933676No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA710.2280391841978481No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA700.22482736470210374No Hit
GCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA670.21519190621487072No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA670.21519190621487072No Hit
CCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA620.19913280873614903No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA610.19592098924040469No Hit
ACCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA600.19270916974466035No Hit
GCCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA600.19270916974466035No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA590.189497350248916No Hit
TCCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA570.18307371125742733No Hit
TCCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA570.18307371125742733No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA520.16701461377870563No Hit
TCCTATGGGGCGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA520.16701461377870563No Hit
ACCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA510.1638027942829613No Hit
GCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA510.1638027942829613No Hit
ACCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA500.16059097478721696No Hit
GCCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA500.16059097478721696No Hit
GCCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA500.16059097478721696No Hit
ACCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA500.16059097478721696No Hit
TCCTACGGGGCGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA490.15737915529147262No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA480.15416733579572828No Hit
CCCTACGGGTGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA470.15095551629998394No Hit
TCCTACGGGGTGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA470.15095551629998394No Hit
TCCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA460.1477436968042396No Hit
TCCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA450.14453187730849526No Hit
GCCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA450.14453187730849526No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGA450.14453187730849526No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.14132005781275092No Hit
GCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA430.13810823831700658No Hit
ACCTACGGGGGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA430.13810823831700658No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA420.13489641882126224No Hit
CCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA410.1316845993255179No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.12526096033402923No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.12526096033402923No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.11883732134254056No Hit
ACCTACGGGGTGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA370.11883732134254056No Hit
ACCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA360.11562550184679621No Hit
CCCTACGGGGTGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA350.11241368235105187No Hit
CCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA350.11241368235105187No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA340.10920186285530754No Hit
CCCTACGGGTGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA340.10920186285530754No Hit
ACCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA330.10599004335956319No Hit
CCCTACGGGGGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA330.10599004335956319No Hit
ACCTATGGGGCGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA330.10599004335956319No Hit
TCCTACGGGAGGCAGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA320.10277822386381885No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA320.10277822386381885No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA320.10277822386381885No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA320.10277822386381885No Hit
CCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA320.10277822386381885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)