FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27585
Sequences flagged as poor quality0
Sequence length281
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2390.8664129055646185No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1980.7177814029363785No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1940.7032807685336233No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1940.7032807685336233No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.6561537067246692No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.6344027551205366No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1700.6162769621170927No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.5691499003081385No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.5618995831067609No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.5401486315026283No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.5220228384991843No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.478520935290919No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.46039514228747513No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.44951966648540875No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.44951966648540875No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.43139387348196484No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.37339133587094436No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1020.36976617727025557No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.35889070146818924No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.34076490846474533No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.33713974986405654No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.33713974986405654No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.3190139568606126No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.3153887982599239No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.3153887982599239No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA860.3117636396592351No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.30813848105854635No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.3045133224578575No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.30088816385716877No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.29726300525648No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.29001268805510244No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.29001268805510244No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.29001268805510244No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.28276237085372485No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.27913721225303606No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.27188689505165853No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.26826173645096973No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.26826173645096973No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.26826173645096973No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.26463657785028094No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.26101141924959215No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA700.2537611020482146No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA700.2537611020482146No Hit
AGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA690.2501359434475258No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.2501359434475258No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.24651078484683706No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA670.24288562624614826No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.2392604676454595No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.2356353090447707No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.2356353090447707No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.2356353090447707No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.2356353090447707No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.2320101504440819No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.2320101504440819No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA630.22838499184339314No Hit
TGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA630.22838499184339314No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.22838499184339314No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA630.22838499184339314No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.22475983324270438No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.2211346746420156No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.2211346746420156No Hit
TGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA600.2175095160413268No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.21388435744063802No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.21388435744063802No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.21388435744063802No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.21025919883994926No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.21025919883994926No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.21025919883994926No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA580.21025919883994926No Hit
TGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA560.2030088816385717No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.2030088816385717No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.2030088816385717No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.19938372303788288No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA540.1957585644371941No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA540.1957585644371941No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.19213340583650534No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA530.19213340583650534No Hit
TGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA530.19213340583650534No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.19213340583650534No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.19213340583650534No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA520.18850824723581658No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.18850824723581658No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA520.18850824723581658No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.18850824723581658No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA510.18488308863512778No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.18125793003443902No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.18125793003443902No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.18125793003443902No Hit
AGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA500.18125793003443902No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.18125793003443902No Hit
CGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA500.18125793003443902No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.17763277143375023No Hit
GGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA490.17763277143375023No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA490.17763277143375023No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.17763277143375023No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.17763277143375023No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.17763277143375023No Hit
AGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA480.17400761283306146No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.17400761283306146No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA480.17400761283306146No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.17400761283306146No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.17038245423237267No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.17038245423237267No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.17038245423237267No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA460.16675729563168387No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.1631321370309951No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.1631321370309951No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.1595069784303063No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.1595069784303063No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.1595069784303063No Hit
TGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA440.1595069784303063No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.15588181982961755No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.15588181982961755No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.15588181982961755No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.15225666122892875No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.15225666122892875No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.14863150262824No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA400.14500634402755122No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.14500634402755122No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA390.14138118542686243No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.14138118542686243No Hit
CGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA380.13775602682617366No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.13775602682617366No Hit
TGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA370.13413086822548487No Hit
GGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA370.13413086822548487No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.13413086822548487No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA370.13413086822548487No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA370.13413086822548487No Hit
AGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA360.13050570962479607No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA360.13050570962479607No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.1268805510241073No Hit
TGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA340.12325539242341853No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA340.12325539242341853No Hit
CGACTACGAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA340.12325539242341853No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA320.11600507522204095No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA310.11237991662135219No Hit
TGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA310.11237991662135219No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA310.11237991662135219No Hit
TGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA300.1087547580206634No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA300.1087547580206634No Hit
AGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA300.1087547580206634No Hit
GGACTACAAGGGTCTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA300.1087547580206634No Hit
CGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA300.1087547580206634No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA290.10512959941997463No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA290.10512959941997463No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA290.10512959941997463No Hit
CGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA280.10150444081928585No Hit
AGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA280.10150444081928585No Hit
GGACTACCAGGGTCTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA280.10150444081928585No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA280.10150444081928585No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA280.10150444081928585No Hit
CGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA280.10150444081928585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)