FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35423
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA17725.002399570900263No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7542.1285605397623013No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA6491.832142957965164No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5241.4792648843971432No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5151.4538576631002456No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4891.3804590237980972No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4721.3324676057928464No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4531.2788301386105074No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4181.1800242780114614No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4011.1320328600062106No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3881.0953335403551365No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3881.0953335403551365No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3721.0501651469384299No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3440.9711204584591931No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3380.9541823109279282No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3270.9231290404539424No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3160.8920757699799565No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3140.8864297208028681No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3050.8610224995059707No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3020.8525534257403381No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3000.8469073765632499No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2910.8215001552663523No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2870.8102080569121758No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2820.7960929339694549No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2740.7735087372611016No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2730.7706857126725574No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2720.7678626880840131No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2720.7678626880840131No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2690.7593936143183807No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2680.7565705897298366No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2670.7537475651412924No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2630.7424554667871157No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.6690568274849674No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.6577647291307908No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2220.6267114586568049No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2160.6097733111255399No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2000.5646049177088333No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.5617818931202891No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.5589588685317449No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.5589588685317449No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.5561358439432007No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.5194365242921266No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1800.5081444259379498No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1770.4996753521723174No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1760.4968523275837733No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1740.4912062784066849No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.4855602292295966No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1710.48273720464105246No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.43192276204725744No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.42345368828162494No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1460.4121615899274482No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1420.4008694915732716No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1420.4008694915732716No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1410.39804646698472745No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.3867543686305508No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1350.38110831945346246No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.35287807356802076No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.35287807356802076No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.35287807356802076No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1180.3331169014482116No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.3331169014482116No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1170.3302938768596675No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1150.3246478276825791No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1150.3246478276825791No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1120.3161787539169466No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.31335572932840244No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.31335572932840244No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.30488665556276995No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1010.2851254834429608No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.27101036050023997No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA960.27101036050023997No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA950.2681873359116958No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA930.26254128673460747No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA910.25689523755751914No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA910.25689523755751914No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA890.2512491883804308No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.24842616379188664No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA870.24560313920334245No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA870.24560313920334245No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.23713406543770998No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.23713406543770998No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA830.2343110408491658No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.23148801626062163No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA820.23148801626062163No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA790.22301894249498913No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.21737289331790077No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA760.21454986872935664No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA750.21172684414081247No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA750.21172684414081247No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.20890381955226833No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.2060807949637241No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA720.20325777037517997No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA690.19478869660954748No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGA690.19478869660954748No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA670.18914264743245915No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA670.18914264743245915No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA660.18631962284391496No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA650.18349659825537082No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA650.18349659825537082No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.17785054907828246No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.17785054907828246No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA630.17785054907828246No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.16938147531265No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.16938147531265No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA600.16938147531265No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA590.1665584507241058No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.16091240154701747No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA570.16091240154701747No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA560.1580893769584733No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA540.15244332778138497No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.14679727860429664No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA510.14397425401575248No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.14397425401575248No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA500.1411512294272083No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.13832820483866415No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.13832820483866415No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA490.13832820483866415No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGA480.13550518025011998No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA470.13268215566157582No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.12985913107303165No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.12985913107303165No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA450.12703610648448746No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA440.12421308189594332No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA440.12421308189594332No Hit
GCCTACGGGAGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA440.12421308189594332No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA420.11856703271885499No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA410.11574400813031081No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.11292098354176663No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.11009795895322248No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA390.11009795895322248No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.10727493436467832No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.10727493436467832No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA370.10445190977613417No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA370.10445190977613417No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA360.10162888518758999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)