FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34598
Sequences flagged as poor quality0
Sequence length321
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA20175.829816752413435No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6681.9307474420486734No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5171.4943060292502457No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA4891.4133764957511996No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4861.4047054743048732No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4811.390253771894329No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4801.3873634314122203No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4531.309324238395283No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4491.2977628764668478No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4491.2977628764668478No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4311.2457367477888897No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4021.1619168738077346No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3681.0636452974160355No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3671.0607549569339267No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3621.046303254523383No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3621.046303254523383No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3601.0405225735591652No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3430.9913867853633159No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3300.9538123590959016No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3300.9538123590959016No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3290.9509220186137927No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3220.9306896352390311No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3090.8931152089716168No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3030.8757731660789642No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2920.8439794207757675No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.8324180588473322No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2870.8295277183652234No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2820.8150760159546794No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2640.7630498872767212No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2560.7399271634198509No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2500.7225851205271981No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.696572056188219No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.6734493323313486No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2250.6503266084744783No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2230.6445459275102607No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.6387652465460432No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.6214232036533903No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.5925197988323024No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1890.5462743511185618No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1860.5376033296722353No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1850.5347129891901267No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1660.4797965200300595No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1610.46534481761951557No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1590.459564136655298No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.4393317532805365No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1500.4335510723163189No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1440.4162090294236661No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA1390.4017573270131221No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1350.390195965084687No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1240.3584022197814902No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1240.3584022197814902No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1200.3468408578530551No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1060.30637609110353203No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1000.28903404821087925No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA960.2774726862824441No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA960.2774726862824441No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA950.2745823458003353No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA930.26880166483611767No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.2659113243540089No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA920.2659113243540089No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.2659113243540089No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA910.2630209838719001No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA900.2601306433897913No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA870.25145962194346494No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.24856928146135612No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.24856928146135612No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA860.24856928146135612No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA820.23700791953292097No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA820.23700791953292097No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA800.23122723856870336No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA790.22833689808659463No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA760.21966587664026824No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.20810451471183306No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.20521417422972427No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA690.1994334932655067No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA660.1907624718191803No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA660.1907624718191803No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA660.1907624718191803No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGA640.18498179085496272No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.18209145037285393No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.18209145037285393No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA610.17631076940863633No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.17342042892652754No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.17342042892652754No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.16185906699809235No Hit
TCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA550.1589687265159836No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.1589687265159836No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA540.1560783860338748No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGA510.1474073645875484No Hit
TCCTACGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA500.14451702410543962No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.14162668362333083No Hit
TCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA490.14162668362333083No Hit
TCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA470.13584600265911323No Hit
ACCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA450.13006532169489565No Hit
ACCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA450.13006532169489565No Hit
GCCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGA450.13006532169489565No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA450.13006532169489565No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.12717498121278686No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.12428464073067806No Hit
GCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA420.12139430024856927No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA420.12139430024856927No Hit
GCCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA410.11850395976646048No Hit
GCCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA410.11850395976646048No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA410.11850395976646048No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA410.11850395976646048No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA400.11561361928435168No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGGAACCCTGA400.11561361928435168No Hit
TCCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.11272327880224292No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA390.11272327880224292No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA390.11272327880224292No Hit
TCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA390.11272327880224292No Hit
ACCTATGGGGCGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA380.10983293832013412No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.10983293832013412No Hit
GCCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA370.10694259783802533No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA360.10405225735591653No Hit
CCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA360.10405225735591653No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGA350.10116191687380774No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA350.10116191687380774No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA350.10116191687380774No Hit
GCCTACGGGACGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.10116191687380774No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT350.10116191687380774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)