FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51471
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA24614.7813331779060055No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA14192.756892230576441No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9511.8476423617182491No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6361.2356472576790816No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6291.222047366478211No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6281.2201045248780866No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6201.204561792077092No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6141.192904742476346No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5661.0996483456703776No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA5411.051077305667269No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5351.0394202560665229No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5050.9811350080627926No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4860.9442210176604302No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4670.9073070272580676No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4590.8917642944570728No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4540.8820500864564512No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4470.8684501952555808No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4470.8684501952555808No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4240.8237648384527209No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4160.8082221056517261No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4140.8043364224514775No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA4100.7965650560509802No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4090.7946222144508559No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4030.7829651648501098No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3780.7343941248470013No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3760.7305084416467525No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3640.7071943424452604No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3620.7033086592450117No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3620.7033086592450117No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3620.7033086592450117No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3550.6897087680441414No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3520.6838802432437684No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3390.658623302442152No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3350.6508519360416546No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3330.6469662528414059No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3230.6275378368401624No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3210.6236521536399137No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3200.6217093120397894No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3080.5983952128382972No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3070.596452371238173No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA3060.5945095296380486No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3010.5847953216374269No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2940.5711954304365565No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2920.5673097472363079No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2890.5614812224359348No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2860.5556526976355618No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2810.5459384896349401No Hit
GCCTACGGGAGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA2760.5362242816343183No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2710.5265100736336966No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2640.5129101824328263No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2560.49736744963183155No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2520.4895960832313341No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2480.4818247168308368No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2470.47988187523071246No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2470.47988187523071246No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2470.47988187523071246No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2450.4759961920304637No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2410.46822482562996637No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2380.4623963008295933No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2350.45656777602922033No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2350.45656777602922033No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2320.4507392512288473No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2300.44685356802859866No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2290.44491072642847435No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2220.4313108352276039No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2140.41576810242660917No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.41382526082648485No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA2050.3982825280254901No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2000.38856832002486835No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1970.38273979522449536No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1960.380796953624371No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1940.3769112704241223No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1910.3710827456237493No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1880.3652542208233763No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1870.36331137922325196No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1780.3458258048221329No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1770.3438829632220085No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.33999728002175983No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1750.33999728002175983No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1680.3263973888208894No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1670.32445454722076505No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1550.301140448019273No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1540.2991976064191486No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1540.2991976064191486No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1520.29531192321889993No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGA1520.29531192321889993No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1520.29531192321889993No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1500.29142624001865125No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1480.2875405568184026No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1340.2603407744166618No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1230.23896951681529408No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1190.23119815041479666No Hit
TCCTATGGGGCGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA1120.21759825921392628No Hit
GCCTATGGGGCGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA960.18651279361193682No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA940.18262711041168814No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA890.17291290241106644No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA880.1709700608109421No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGA870.16902721921081776No Hit
TCCTACGGGTGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA850.16514153601056905No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA850.16514153601056905No Hit
ACCTATGGGGCGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA830.16125585281032037No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA800.15542732800994735No Hit
TCCTACGGGGCGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA770.1495988032095743No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA750.14571312000932563No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA730.14182743680907697No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA700.13599891200870393No Hit
CCCTATGGGGCGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA690.1340560704085796No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA690.1340560704085796No Hit
TCCTACGGGAGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA670.1301703872083309No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA650.12628470400808223No Hit
ACCTACGGGAGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA640.12434186240795789No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA630.12239902080783352No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA620.1204561792077092No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA610.11851333760758485No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA610.11851333760758485No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA590.11462765440733617No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA590.11462765440733617No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA580.11268481280721182No Hit
TCCTACGGGTGGCAGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA580.11268481280721182No Hit
GCCTACGGGAGGCAGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA570.11074197120708748No Hit
TCCTATGGGGCGCAGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA570.11074197120708748No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA560.10879912960696314No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA540.10491344640671448No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA540.10491344640671448No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA530.10297060480659011No Hit
GCCTACGGGTGGCTGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA530.10297060480659011No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA530.10297060480659011No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA520.10102776320646577No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGTAGCCTGA520.10102776320646577No Hit
ACCTACGGGAGGCAGCAGTAGGGAGTATTTGGCAATGGGGGAAACCCTGA520.10102776320646577No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA520.10102776320646577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)