FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17260
Sequences flagged as poor quality0
Sequence length321
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA7974.617612977983778No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3301.9119351100811124No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA3251.8829663962920047No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2311.3383545770567786No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2291.3267670915411356No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2061.19351100811124No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2021.1703360370799536No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1881.089223638470452No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1720.996523754345307No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1720.996523754345307No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1630.9443800695249132No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1620.9385863267670916No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1600.9269988412514484No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1560.9038238702201622No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1560.9038238702201622No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1420.8227114716106605No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1380.7995365005793743No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1370.7937427578215528No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1370.7937427578215528No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1350.7821552723059096No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1320.764774044032445No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1300.7531865585168018No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1300.7531865585168018No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1250.7242178447276941No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1250.7242178447276941No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1240.7184241019698725No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1230.712630359212051No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1230.712630359212051No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1210.7010428736964079No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1200.6952491309385863No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1190.6894553881807648No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1180.6836616454229432No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1130.6546929316338355No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1080.6257242178447276No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1080.6257242178447276No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1020.5909617612977984No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1010.5851680185399768No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1000.5793742757821553No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA960.5561993047508691No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.544611819235226No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA920.5330243337195829No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA900.5214368482039398No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.5156431054461181No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.5156431054461181No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA880.5098493626882966No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA850.49246813441483195No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA830.4808806488991889No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA790.4577056778679027No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA790.4577056778679027No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.41714947856315177No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA700.4055619930475087No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA690.39976825028968715No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.3939745075318656No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA680.3939745075318656No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA670.38818076477404406No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA640.3707995365005794No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA630.3650057937427578No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA610.35341830822711473No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA600.34762456546929316No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.3418308227114716No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA590.3418308227114716No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA590.3418308227114716No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.33603707995365006No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA570.3302433371958285No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA560.3244495944380069No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA550.3186558516801854No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA530.30706836616454225No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA510.2954808806488992No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.27809965237543455No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA480.27809965237543455No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.272305909617613No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA470.272305909617613No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA470.272305909617613No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.26651216685979146No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.26651216685979146No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.26651216685979146No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA460.26651216685979146No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA450.2607184241019699No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA450.2607184241019699No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.24913093858632676No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.24913093858632676No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA430.24913093858632676No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA420.24333719582850522No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.24333719582850522No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.24333719582850522No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA410.23754345307068367No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA400.2317497103128621No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA390.22595596755504055No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA390.22595596755504055No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA380.220162224797219No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA360.20857473928157588No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA350.20278099652375434No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA350.20278099652375434No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA350.20278099652375434No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA340.1969872537659328No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA320.1853997682502897No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA320.1853997682502897No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.17960602549246812No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA300.17381228273464658No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA290.16801853997682503No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.16801853997682503No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA290.16801853997682503No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA280.16222479721900346No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA270.1564310544611819No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA270.1564310544611819No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA270.1564310544611819No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.1506373117033604No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.1506373117033604No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA260.1506373117033604No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA250.14484356894553882No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA250.14484356894553882No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA250.14484356894553882No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA240.13904982618771727No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA240.13904982618771727No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA240.13904982618771727No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGA230.13325608342989573No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA230.13325608342989573No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA230.13325608342989573No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA230.13325608342989573No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA230.13325608342989573No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA230.13325608342989573No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA230.13325608342989573No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA220.12746234067207415No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA220.12746234067207415No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA210.12166859791425261No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA210.12166859791425261No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGA210.12166859791425261No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA210.12166859791425261No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA210.12166859791425261No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA200.11587485515643105No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA200.11587485515643105No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA200.11587485515643105No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA200.11587485515643105No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA200.11587485515643105No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA200.11587485515643105No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA200.11587485515643105No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA200.11587485515643105No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA200.11587485515643105No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA200.11587485515643105No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA190.1100811123986095No Hit
TCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA190.1100811123986095No Hit
GCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA190.1100811123986095No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA190.1100811123986095No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA190.1100811123986095No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA190.1100811123986095No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.10428736964078794No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.10428736964078794No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA180.10428736964078794No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.10428736964078794No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA180.10428736964078794No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA180.10428736964078794No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA180.10428736964078794No Hit
TCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.10428736964078794No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA180.10428736964078794No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA180.10428736964078794No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA180.10428736964078794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)