FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26018
Sequences flagged as poor quality0
Sequence length321
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA15205.842109308939965No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6122.352217695441617No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4381.6834499192866477No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4351.671919440387424No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4081.5681451302944116No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4041.55277115842878No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA3831.4720578061342149No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3731.433622876470136No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3461.3298485663771236No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3351.287570143746637No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3221.2376047351833346No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3111.195326312552848No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3051.1722653547544009No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3041.168421861787993No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2991.1492043969559536No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2961.13767391805673No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2691.0338996079637175No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2621.0069951571988625No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2621.0069951571988625No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2621.0069951571988625No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2611.0031516642324545No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2590.9954646782996388No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2580.9916211853332308No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2490.95702974863556No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2460.9454992697363364No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.9416557767699285No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2410.926281804904297No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.895533861173034No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2140.8225074948112846No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2110.810977015912061No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2020.77638557921439No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2000.7686985932815743No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.7648551003151665No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.7533246214159428No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1900.7302636636174956No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1830.7033592128526405No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1780.6841417480206011No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.6726112691213775No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.6533938042893381No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA1680.6457068183565224No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1650.6341763394572988No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.6188023675916673No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.6188023675916673No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1500.5765239449611808No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1310.5034975785994312No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1260.48428011376739183No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1170.44968867706972093No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1160.4458451841033131No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1110.4266277192712737No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1070.4112537474056423No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA930.35744484587593206No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA910.3497578599431163No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA910.3497578599431163No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA910.3497578599431163No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA900.3459143669767084No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.3228534091782612No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA810.3113229302790376No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA800.3074794373126297No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.299792451379814No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA770.2959489584134061No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.2921054654469982No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA750.2882619724805904No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.2844184795141825No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.2844184795141825No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA720.2767314935813667No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.2728880006149589No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.2728880006149589No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.2728880006149589No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA700.269044507648551No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.26520101468214313No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.26520101468214313No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA690.26520101468214313No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA650.24982704281651166No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.2459835498501038No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.23829656391728804No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.23829656391728804No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA580.22292259205165654No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA570.21907909908524867No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.2152356061188408No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA550.21139211315243292No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA540.20754862018602505No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA530.2037051272196172No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.2037051272196172No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.2037051272196172No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA520.19986163425320932No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.19601814128680145No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.19217464832039358No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA500.19217464832039358No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA470.18064416942116995No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA470.18064416942116995No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.1729571834883542No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA440.16911369052194633No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA440.16911369052194633No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA440.16911369052194633No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA440.16911369052194633No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA430.16527019755553846No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA430.16527019755553846No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA420.1614267045891306No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA420.1614267045891306No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA410.15758321162272274No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA410.15758321162272274No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA410.15758321162272274No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA400.15373971865631486No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA400.15373971865631486No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA390.149896225689907No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA380.1460527327234991No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA360.13836574679068336No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.13836574679068336No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.13836574679068336No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA360.13836574679068336No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA360.13836574679068336No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA350.1345222538242755No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA340.13067876085786764No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA340.13067876085786764No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGA330.12683526789145977No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA330.12683526789145977No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA320.1229917749250519No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.1229917749250519No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.1229917749250519No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA320.1229917749250519No Hit
TCCTATGGGGCGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA310.11914828195864402No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.11914828195864402No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.11914828195864402No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA310.11914828195864402No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA310.11914828195864402No Hit
TCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA290.11146129602582827No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11146129602582827No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11146129602582827No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11146129602582827No Hit
GCCTATGGGGCGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA290.11146129602582827No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11146129602582827No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA290.11146129602582827No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11146129602582827No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.11146129602582827No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA280.1076178030594204No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA280.1076178030594204No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAAAGTCTGA270.10377431009301252No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGA270.10377431009301252No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA270.10377431009301252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)