FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48122
Sequences flagged as poor quality0
Sequence length321
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA27815.779061551888949No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9491.9720709862432981No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7161.487884959062383No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7151.485806907443581No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6871.427621462117119No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6581.3673579651718548No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6171.2821578488009642No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5991.2447529196625244No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5811.2073479905240845No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5781.201113835667678No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5631.169943061385645No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5441.1304600806284029No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5371.1159137192967874No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5371.1159137192967874No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5061.0514941191139187No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4891.016167241594281No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4720.9808403640746435No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4700.9766842608370392No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4670.9704501059806325No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4630.9621378995054237No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4430.9205768671293795No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4400.9143427122729728No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4380.9101866090353685No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4270.8873280412285441No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4240.8810938863721375No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4230.8790158347533352No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4190.8707036282781265No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4170.866547525040522No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4130.8582353185653131No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3950.8208303894268734No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3900.8104401313328622No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3730.7751132538132247No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3540.7356302730559827No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3470.7210839117243673No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3200.6649765180167075No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA3050.6338057437346744No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2900.6026349694526413No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2880.5984788662150368No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2820.5860105565022234No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2740.5693861435518058No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2700.561073937076597No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2380.4945762852749262No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA2250.46756161423049747No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA2050.4260005818544532No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2050.4260005818544532No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2020.4197664269980466No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.3761273430032002No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1770.36781513652799136No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1490.30962969120152944No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1410.2930052782511117No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1220.2535222974938698No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA1180.24521009101866087No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1170.2431320393998587No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGA1070.2223515232118366No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA1020.21196126511782554No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA920.1911807489298034No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA910.1891026973110012No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA880.18286854245459458No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAAGCCTGA820.17040023274178132No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA800.16624412950417689No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA780.16208802626657245No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA770.16000997464777025No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA750.15585387141016585No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA750.15585387141016585No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA740.15377581979136362No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA730.1516977681725614No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA720.1496197165537592No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA700.1454636133161548No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA660.13715140684094593No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA660.13715140684094593No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA660.13715140684094593No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA650.13507335522214373No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA640.1329953036033415No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.1288392003657371No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA610.1267611487469349No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA590.12260504550933043No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA590.12260504550933043No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA590.12260504550933043No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA580.12052699389052825No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA570.11844894227172602No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA570.11844894227172602No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA560.11637089065292383No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA560.11637089065292383No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA560.11637089065292383No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA550.1142928390341216No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA520.10805868417771497No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA520.10805868417771497No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA510.10598063255891277No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA500.10390258094011054No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA490.10182452932130835No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA490.10182452932130835No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCCTGA490.10182452932130835No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA490.10182452932130835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)