FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27037
Sequences flagged as poor quality0
Sequence length321
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9193.399045752117469No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA4301.590413137552243No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2941.0873987498613011No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.9135628952916374No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2430.8987683544771979No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2310.8543847320338795No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2210.8173983799977809No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2150.7952065687761215No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2130.7878092983689019No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2030.7508229463328032No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2020.7471243111291933No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1860.6879461478714355No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1820.673151607056996No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.6546584310389466No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.6472611606317269No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1700.6287679846136776No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.6250693494100676No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1630.6028775381884084No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA1600.5917816325775789No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.584384362170359No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1540.5695898213559197No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1530.5658911861523097No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1470.5436993749306506No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1460.5400007397270408No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1410.5215075637089914No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.5215075637089914No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1400.5178089285053815No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1390.5141102933017717No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.5067130228945519No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.5067130228945519No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.5067130228945519No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1330.4919184820801124No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1330.4919184820801124No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1290.477123941265673No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1270.46972667085845327No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.46602803565484335No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1190.44013758922957436No Hit
TCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1190.44013758922957436No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA1130.41794577800791505No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1110.41054850760069533No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1090.40315123719347556No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.39945260198986576No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1050.38835669637903614No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1050.38835669637903614No Hit
GCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1030.3809594259718164No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA1000.3698635203609868No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA990.36616488515737694No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA970.35876761475015717No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.3550689795465473No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.3550689795465473No Hit
ACCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA960.3550689795465473No Hit
TCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA910.336575803528498No Hit
TCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA910.336575803528498No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA870.3217812627140585No Hit
TCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA840.3106853571032289No Hit
ACCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA840.3106853571032289No Hit
CCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA810.2995894514923993No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGA810.2995894514923993No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA800.29589081628878944No Hit
GCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA800.29589081628878944No Hit
TCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA790.2921921810851795No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA790.2921921810851795No Hit
ACCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA780.2884935458815697No Hit
ACCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA780.2884935458815697No Hit
TCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA780.2884935458815697No Hit
GCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA770.28479491067795987No Hit
CCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA760.28109627547434995No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA760.28109627547434995No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA760.28109627547434995No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA740.2736990050671302No Hit
GCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA720.2663017346599105No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA720.2663017346599105No Hit
GCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA720.2663017346599105No Hit
GCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA710.2626030994563006No Hit
ACCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA700.25890446425269076No Hit
GCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA690.2552058290490809No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA680.251507193845471No Hit
CCCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA680.251507193845471No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA670.24780855864186116No Hit
ACCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA670.24780855864186116No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGA670.24780855864186116No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.24041128823464142No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.23671265303103153No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.23301401782742168No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA620.22931538262381182No Hit
GCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGA620.22931538262381182No Hit
GCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA590.2182194770129822No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.21452084180937234No Hit
CCCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA580.21452084180937234No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.21452084180937234No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGA570.21082220660576245No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.20712357140215262No Hit
CCCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA560.20712357140215262No Hit
CCCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA540.19972630099493288No Hit
TCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA540.19972630099493288No Hit
CCCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA530.196027665791323No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.196027665791323No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.196027665791323No Hit
CCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA530.196027665791323No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA520.19232903058771314No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.18863039538410326No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA510.18863039538410326No Hit
ACCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA500.1849317601804934No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.18123312497688354No Hit
ACCTACGGGGTGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA480.17753448977327366No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.17753448977327366No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA470.1738358545696638No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGAAACCCTGA460.17013721936605392No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.17013721936605392No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.17013721936605392No Hit
CCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA450.16643858416244406No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA440.1627399489588342No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.1627399489588342No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA440.1627399489588342No Hit
TCCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA430.15904131375522432No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA430.15904131375522432No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.14794540814439472No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA400.14794540814439472No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA390.14424677294078486No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA390.14424677294078486No Hit
ACCTACGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA390.14424677294078486No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.14054813773717498No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.14054813773717498No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.14054813773717498No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA380.14054813773717498No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA380.14054813773717498No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.1368495025335651No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.1368495025335651No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.1368495025335651No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.1368495025335651No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.1368495025335651No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA370.1368495025335651No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA360.13315086732995526No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.12945223212634538No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA340.1257535969227355No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.1257535969227355No Hit
ACCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA340.1257535969227355No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.12205496171912565No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.12205496171912565No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA330.12205496171912565No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA330.12205496171912565No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.11835632651551577No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA320.11835632651551577No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGA320.11835632651551577No Hit
GCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA320.11835632651551577No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.11835632651551577No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA310.11465769131190591No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.11465769131190591No Hit
ACCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA310.11465769131190591No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.11465769131190591No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA310.11465769131190591No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA310.11465769131190591No Hit
CCCTATGGGGCGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGA310.11465769131190591No Hit
TCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA300.11095905610829604No Hit
TCCTACGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA300.11095905610829604No Hit
GCCTATGGGGCGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA300.11095905610829604No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG290.10726042090468617No Hit
TCCTATGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGA290.10726042090468617No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA290.10726042090468617No Hit
TCCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA290.10726042090468617No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA290.10726042090468617No Hit
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA290.10726042090468617No Hit
TCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA280.10356178570107631No Hit
TCCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA280.10356178570107631No Hit
ACCTACGGGGCGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGA280.10356178570107631No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA280.10356178570107631No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA280.10356178570107631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)