FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60237
Sequences flagged as poor quality0
Sequence length321
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA35105.8269834155087405No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA10121.6800305460099276No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8771.4559157992595912No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8451.4027923037335857No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8361.3878513206168965No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA8241.3679300097946445No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7601.2616830187426331No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7141.1853179939240002No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7101.1786775569832495No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6831.1338546076331824No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6831.1338546076331824No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6761.1222338429868686No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6161.022627288875608No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6131.017646961170045No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6111.0143267426996696No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5830.9678436841144148No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5790.961203247173664No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5790.961203247173664No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5710.9479223732921627No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5560.9230207347643475No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5530.9180404070587844No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5470.9080797516476583No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5410.8981190962365323No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA5390.894798877766157No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5280.8765376761790925No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5050.838355163769776No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4840.8034928698308349No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4840.8034928698308349No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4670.7752710128326443No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4510.7487092650696416No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA4480.7437289373640785No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4370.7254677357770141No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4220.700566097249199No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4160.6906054418380729No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4140.6872852233676976No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4140.6872852233676976No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3780.6275212909009412No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3500.5810382323156863No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3490.5793781230804986No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3350.5561365937878713No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3160.5245945183193054No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3100.5146338629081794No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA3050.506333316732241No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2930.48641200590998884No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2690.44656938426548465No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2510.41668741803210657No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA2350.3901256702691037No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA2130.35360326709497486No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1970.32704151933197206No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1870.3104404269800953No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA1750.29051911615784315No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1750.29051911615784315No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA1330.22079452827996082No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGA1320.21913441904477313No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1280.21249398210402243No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1260.20917376363364712No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1250.20751365439845942No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCGAGCCTGA1240.20585354516327176No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1230.2041934359280841No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1220.2025333266928964No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1210.20087321745770872No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1170.194232780516958No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1160.19257267128177033No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1130.18759234357620733No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1110.18427212510583196No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA1080.17929179740026896No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1080.17929179740026896No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1070.17763168816508126No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1060.1759715789298936No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1060.1759715789298936No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1050.1743114696947059No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA1040.17265136045951823No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1030.17099125122433056No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGTCAATGGGGGGAACCCTGA1020.16933114198914287No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1010.1676710327539552No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA1010.1676710327539552No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA990.16435081428357987No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA980.1626907050483922No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA970.1610305958132045No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA930.15439015887245383No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA930.15439015887245383No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA900.14940983116689077No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA890.1477497219317031No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA890.1477497219317031No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA880.14608961269651544No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA880.14608961269651544No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA860.14276939422614007No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA860.14276939422614007No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA860.14276939422614007No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGA850.1411092849909524No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA850.1411092849909524No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA850.1411092849909524No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA850.1411092849909524No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.13944917575576474No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA840.13944917575576474No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA830.13778906652057707No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA820.13612895728538937No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA810.1344688480502017No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA800.132808738815014No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA790.13114862957982634No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA790.13114862957982634No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA780.12948852034463867No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA780.12948852034463867No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.12948852034463867No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA780.12948852034463867No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA750.12450819263907566No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA740.12284808340388798No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA740.12284808340388798No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA730.1211879741687003No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA720.11952786493351261No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA720.11952786493351261No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA720.11952786493351261No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA710.11786775569832494No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA710.11786775569832494No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA710.11786775569832494No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA700.11620764646313728No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA700.11620764646313728No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA700.11620764646313728No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA700.11620764646313728No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA700.11620764646313728No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.11454753722794961No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA690.11454753722794961No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA690.11454753722794961No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.11122731875757425No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.11122731875757425No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.11122731875757425No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.11122731875757425No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA670.11122731875757425No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA670.11122731875757425No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA660.10956720952238656No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA650.1079071002871989No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA640.10624699105201121No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA640.10624699105201121No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA640.10624699105201121No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGA630.10458688181682356No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA630.10458688181682356No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA620.10292677258163588No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA620.10292677258163588No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA620.10292677258163588No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA610.1012666633464482No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA610.1012666633464482No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA610.1012666633464482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)