FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25776
Sequences flagged as poor quality0
Sequence length321
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA8093.1385785226567346No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7783.0183116076970826No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2540.9854127870887648No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2360.915580384854128No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.8108317815021726No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2090.8108317815021726No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2040.7914338919925512No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2000.7759155803848541No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2000.7759155803848541No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1950.7565176908752328No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1940.7526381129733085No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.702203600248293No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1810.702203600248293No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1810.702203600248293No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1800.6983240223463687No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1770.6866852886405959No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1750.6789261328367473No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1720.6672873991309746No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1710.6634078212290503No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1680.6517690875232774No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1670.6478895096213532No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.6478895096213532No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1670.6478895096213532No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.6478895096213532No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1580.6129733085040348No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1560.6052141527001862No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1520.5896958410924891No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1520.5896958410924891No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1500.5819366852886406No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1480.5741775294847921No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1470.5702979515828678No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1470.5702979515828678No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1430.5547796399751708No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA1430.5547796399751708No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.5547796399751708No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1430.5547796399751708No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1410.5470204841713222No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1390.5392613283674736No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.5315021725636251No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1370.5315021725636251No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1370.5315021725636251No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1360.5276225946617008No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1360.5276225946617008No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1350.5237430167597765No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1350.5237430167597765No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1330.515983860955928No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1320.5121042830540037No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1300.5043451272501551No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1300.5043451272501551No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1290.500465549348231No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1280.4965859714463066No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1270.4927063935443824No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1260.4888268156424581No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1250.48494723774053383No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1240.48106765983860955No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.46942892613283677No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1200.4655493482309125No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1160.45003103662321536No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1150.4461514587212911No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1130.4383923029174426No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.4345127250155183No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1090.4228739913097455No Hit
CCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.41899441340782123No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1080.41899441340782123No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1080.41899441340782123No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1060.41123525760397267No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1030.3995965238981999No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1000.38795779019242704No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA990.38407821229050276No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA970.37631905648665426No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA960.37243947858473No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA940.36468032278088147No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA930.3608007448789572No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.3569211669770329No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA920.3569211669770329No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA920.3569211669770329No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA900.34916201117318435No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA890.34528243327126007No Hit
ACCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA880.3414028553693358No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA860.3336436995654873No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA850.329764121663563No Hit
CCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.3258845437616387No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA780.3026070763500931No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA780.3026070763500931No Hit
CCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA680.2638112973308504No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.25217256362507756No Hit
GCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.24441340782122906No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA620.24053382991930478No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA610.23665425201738052No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA600.23277467411545624No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA580.22501551831160768No Hit
ACCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.21725636250775915No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA560.21725636250775915No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGCGAAAGCCTGA520.20173805090006208No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGA510.1978584729981378No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA510.1978584729981378No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA510.1978584729981378No Hit
CCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA490.19009931719428927No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA470.18234016139044074No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA440.1707014276846679No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA430.16682184978274364No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA420.16294227188081936No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA400.15518311607697083No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA390.15130353817504655No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA370.14354438237119801No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.1319056486654252No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA340.1319056486654252No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA330.12802607076350092No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA330.12802607076350092No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA330.12802607076350092No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA320.12414649286157665No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA320.12414649286157665No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGA320.12414649286157665No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.12026691495965239No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA310.12026691495965239No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA310.12026691495965239No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA310.12026691495965239No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA310.12026691495965239No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA300.11638733705772812No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA300.11638733705772812No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA300.11638733705772812No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11250775915580384No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA290.11250775915580384No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA290.11250775915580384No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA280.10862818125387957No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA280.10862818125387957No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA280.10862818125387957No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA280.10862818125387957No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.10474860335195531No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA270.10474860335195531No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA270.10474860335195531No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGA270.10474860335195531No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA270.10474860335195531No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA270.10474860335195531No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA270.10474860335195531No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA270.10474860335195531No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA260.10086902545003104No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA260.10086902545003104No Hit
CCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA260.10086902545003104No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA260.10086902545003104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)