FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15051
Sequences flagged as poor quality0
Sequence length281
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1821.2092219786060727No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1731.1494252873563218No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1490.9899674440236529No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1450.9633911368015413No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1390.9235266759683742No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1380.9168825991628463No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.9102385223573185No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1360.9035944455517906No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1270.8437977543020397No Hit
CGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.7906451398578167No Hit
AGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.6843399109693709No Hit
CGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA990.6577636037472593No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.62454321971962No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.6112550661085643No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.5647465284698691No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.5448142980532855No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.5381702212477576No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.5248820676367019No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.5182379908311741No Hit
AGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA740.4916616836090625No Hit
TGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.4850176068035347No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA720.47837352999800675No Hit
TGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA710.47172945319247894No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.45844129958142316No Hit
TGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.4517972227758953No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.4517972227758953No Hit
GGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.4385090691648395No Hit
TGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA660.4385090691648395No Hit
TGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.4318649923593117No Hit
GGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.4318649923593117No Hit
GGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA640.4252209155537838No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.4185768387482559No Hit
TGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.4185768387482559No Hit
GGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.4185768387482559No Hit
GGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.41193276194272804No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.41193276194272804No Hit
AGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.4052886851372002No Hit
GGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA610.4052886851372002No Hit
GGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA600.39864460833167237No Hit
TGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.39200053152614445No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.39200053152614445No Hit
GGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.3720683011095608No Hit
GGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.3720683011095608No Hit
TGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.3720683011095608No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.36542422430403293No Hit
AGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.36542422430403293No Hit
TGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA540.35878014749850506No Hit
CGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA530.3521360706929772No Hit
TGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.34549199388744933No Hit
AGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA520.34549199388744933No Hit
TGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA510.33884791708192147No Hit
TGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.3322038402763936No Hit
TGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA500.3322038402763936No Hit
CGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.32555976347086574No Hit
AGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA490.32555976347086574No Hit
TGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA490.32555976347086574No Hit
TGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA480.31891568666533787No Hit
AGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.31891568666533787No Hit
TGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA480.31891568666533787No Hit
TGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA470.31227160985981No Hit
TGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA460.30562753305428214No Hit
TGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA460.30562753305428214No Hit
CGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA460.30562753305428214No Hit
GGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA450.2989834562487542No Hit
AGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.29233937944322635No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA440.29233937944322635No Hit
GGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA440.29233937944322635No Hit
GGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.2856953026376985No Hit
TGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.2856953026376985No Hit
AGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA430.2856953026376985No Hit
AGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.2790512258321706No Hit
CGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.2790512258321706No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA420.2790512258321706No Hit
CGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA420.2790512258321706No Hit
AGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.2790512258321706No Hit
AGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA420.2790512258321706No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.27240714902664276No Hit
CGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.27240714902664276No Hit
CGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.27240714902664276No Hit
AGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.2657630722211149No Hit
CGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA400.2657630722211149No Hit
AGAATACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.25911899541558703No Hit
TGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.25911899541558703No Hit
AGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.25911899541558703No Hit
AGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA390.25911899541558703No Hit
AGACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA390.25911899541558703No Hit
TGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA380.2524749186100591No Hit
TGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.2524749186100591No Hit
CGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA380.2524749186100591No Hit
CGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.24583084180453124No Hit
AGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA370.24583084180453124No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA370.24583084180453124No Hit
CGACTACAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA370.24583084180453124No Hit
CGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA360.23918676499900338No Hit
CGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA360.23918676499900338No Hit
GGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA360.23918676499900338No Hit
CGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.2325426881934755No Hit
CGACTACTAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA350.2325426881934755No Hit
AGACTACACGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.22589861138794765No Hit
AGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.22589861138794765No Hit
CGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA320.2126104577768919No Hit
TGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA320.2126104577768919No Hit
CGACTACTCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA320.2126104577768919No Hit
CGACTACGAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA310.20596638097136402No Hit
AGACTACCGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA310.20596638097136402No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA310.20596638097136402No Hit
AGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA310.20596638097136402No Hit
AGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA310.20596638097136402No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA310.20596638097136402No Hit
CGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA310.20596638097136402No Hit
TGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA300.19932230416583618No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA300.19932230416583618No Hit
CGACTACTGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA300.19932230416583618No Hit
AGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA300.19932230416583618No Hit
CGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA290.1926782273603083No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA280.1860341505547804No Hit
AGACTACGAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA280.1860341505547804No Hit
AGACTACGCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA270.17939007374925253No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA260.17274599694372467No Hit
CGACTACGGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA260.17274599694372467No Hit
CGACTACCAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA250.1661019201381968No Hit
CGACTACAGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA250.1661019201381968No Hit
TGACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA240.15945784333266894No Hit
CGACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA230.15281376652714107No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA230.15281376652714107No Hit
GGACTACTGGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA230.15281376652714107No Hit
AGACTACCCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA230.15281376652714107No Hit
TGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA230.15281376652714107No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA200.13288153611055745No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA200.13288153611055745No Hit
GGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA200.13288153611055745No Hit
TGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA200.13288153611055745No Hit
AGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA200.13288153611055745No Hit
TGACTACTGGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA200.13288153611055745No Hit
TGACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA200.13288153611055745No Hit
TGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA190.12623745930502955No Hit
TGACTACAAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA190.12623745930502955No Hit
TGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA190.12623745930502955No Hit
TGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA190.12623745930502955No Hit
CGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA190.12623745930502955No Hit
AGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA190.12623745930502955No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA190.12623745930502955No Hit
TGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA190.12623745930502955No Hit
AGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA190.12623745930502955No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA190.12623745930502955No Hit
CGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA190.12623745930502955No Hit
CGACTACGCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA180.11959338249950169No Hit
CGACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
GGACTACAGGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
GGACTACGAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
TGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA180.11959338249950169No Hit
TGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA180.11959338249950169No Hit
AGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA180.11959338249950169No Hit
CGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA180.11959338249950169No Hit
CGACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA170.11294930569397382No Hit
TGACTACTAGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA170.11294930569397382No Hit
CGAATACCGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA170.11294930569397382No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA170.11294930569397382No Hit
TGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA170.11294930569397382No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA160.10630522888844594No Hit
GGAATACCGGGGTTTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA160.10630522888844594No Hit
CGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA160.10630522888844594No Hit
TGACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA160.10630522888844594No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA160.10630522888844594No Hit
GGACTACACGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA160.10630522888844594No Hit
GGACTACCCGGGTCTCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA160.10630522888844594No Hit
CGACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCA160.10630522888844594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)