FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008092977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008092977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44097
Sequences flagged as poor quality0
Sequence length321
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA29496.687529763929518No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA9632.183822028709436No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7381.6735832369548949No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA7351.6667800530648345No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6991.5851418463841078No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6661.5103068235934416No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6421.4558813524729572No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6271.4218654330226546No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6251.4173299770959475No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA6101.3833140576456449No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5691.2903372111481506No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5561.260856747624555No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5481.2427149239177269No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5481.2427149239177269No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5441.2336440120643128No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5171.1724153570537679No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5091.1542735333469396No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA5061.1474703494568792No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4961.124793069823344No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4921.11572215796993No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4891.1089189740798695No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4851.0998480622264555No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4811.0907771503730412No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4601.0431548631426173No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4591.040887135179264No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4571.036351679252557No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4491.0182098555457286No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4320.9796584801687189No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4210.9547134725718304No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4200.9524457446084769No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3830.8685398099643967No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA3770.8549334421842756No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3740.8481302582942151No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3550.8050434269904982No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3400.7710275075401954No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3340.7574211397600744No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3320.7528856838333674No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3230.7324761321631857No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3150.7143343084563576No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2970.6735152051159943No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA2790.6326961017756311No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.5782706306551466No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2450.5555933510216114No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA2430.5510578950949043No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA2060.4671519604508243No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1990.45127786470734976No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1960.4444746808172892No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1930.4376714969272286No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1650.3741751139533302No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA1480.3356237385763204No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA1190.26985962763906846No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA1140.2585209878223008No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA950.21543415651858402No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA940.2131664285552305No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA880.19956006077510943No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA850.19275687688504886No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA840.19048914892169536No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA840.19048914892169536No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA810.1836859650316348No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA810.1836859650316348No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA800.18141823706828128No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA760.17234732521486723No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA760.17234732521486723No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA760.17234732521486723No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA740.1678118692881602No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA740.1678118692881602No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA740.1678118692881602No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA730.1655441413248067No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA720.16327641336145315No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA710.16100868539809965No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA710.16100868539809965No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA700.15874095743474612No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA680.1542055015080391No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA670.1519377735446856No Hit
ACCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA670.1519377735446856No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA660.14967004558133207No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA640.14513458965462503No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA630.1428668616912715No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA630.1428668616912715No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA620.140599133727918No Hit
TCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.13606367780121098No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA600.13606367780121098No Hit
CCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA600.13606367780121098No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA600.13606367780121098No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA590.13379594983785745No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.13379594983785745No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA590.13379594983785745No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.1292604939111504No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA570.1292604939111504No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA570.1292604939111504No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGGAGCCTGA570.1292604939111504No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA560.1269927659477969No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA560.1269927659477969No Hit
CCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA560.1269927659477969No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA560.1269927659477969No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA560.1269927659477969No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA560.1269927659477969No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA550.12472503798444337No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA550.12472503798444337No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA550.12472503798444337No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA550.12472503798444337No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA540.12245731002108987No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA540.12245731002108987No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.12018958205773636No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA530.12018958205773636No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.12018958205773636No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA530.12018958205773636No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA530.12018958205773636No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA530.12018958205773636No Hit
ACCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA510.11565412613102931No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA510.11565412613102931No Hit
ACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.11565412613102931No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.11565412613102931No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA510.11565412613102931No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA510.11565412613102931No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.1133863981676758No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA500.1133863981676758No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA500.1133863981676758No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGA490.1111186702043223No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA490.1111186702043223No Hit
GCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA480.10885094224096878No Hit
CCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGA480.10885094224096878No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA470.10658321427761525No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA470.10658321427761525No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA460.10431548631426174No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.10431548631426174No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGA460.10431548631426174No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGA450.10204775835090824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)