FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008093045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008093045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11852
Sequences flagged as poor quality0
Sequence length321
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG11079.340195747553155No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG3623.0543368207897403No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2892.438407019912251No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2872.4215322308471143No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2642.2274721565980427No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2502.1093486331420856No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2502.1093486331420856No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2402.0249746878164023No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2341.9743503206209922No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2211.864664191697604No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2171.8309146135673304No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2151.8140398245021938No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2051.7296658791765105No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2051.7296658791765105No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2051.7296658791765105No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG2031.7127910901113736No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1951.645291933850827No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1911.6115423557205535No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1891.5946675666554166No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1891.5946675666554166No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1841.5524805939925752No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1841.5524805939925752No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1781.5018562267971651No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1741.4681066486668917No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1691.42591967600405No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1681.4174822814714816No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1651.3921700978737768No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1651.3921700978737768No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1641.3837327033412081No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1541.2993587580155248No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1531.2909213634829564No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1411.1896726290921362No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1371.155923050961863No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1361.1474856564292946No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1341.130610867364158No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1241.0462369220384746No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1231.0377995275059062No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1211.0209247384407694No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1100.9281133985825177No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG1010.8521768477894027No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG930.7846776915288558No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA830.7003037462031725No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG810.6834289571380358No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG800.6749915626054674No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTG560.47249409382382723No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA380.320620992237597No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA290.24468444144448193No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA280.23624704691191362No Hit
CCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA260.21937225784677694No Hit
TCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA250.21093486331420858No Hit
GCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA240.20249746878164024No Hit
GCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA200.16874789065136686No Hit
TCCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA190.1603104961187985No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA190.1603104961187985No Hit
TCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA190.1603104961187985No Hit
ACCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA190.1603104961187985No Hit
ACCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
CCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
TCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
ACCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
ACCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
GCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA180.15187310158623016No Hit
CCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA170.14343570705366182No Hit
GCCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA160.13499831252109348No Hit
GCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA160.13499831252109348No Hit
TCCTATGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA160.13499831252109348No Hit
GCCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA150.12656091798852515No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA150.12656091798852515No Hit
GCCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
TCCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
TCCTACGGGGGCTGCAGTGGGGAATATTGGACAATGGACCAAAAGTCTGA140.11812352345595681No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA140.11812352345595681No Hit
GCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
TCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
ACCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
TCCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA140.11812352345595681No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA130.10968612892338847No Hit
ACCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA130.10968612892338847No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA120.10124873439082012No Hit
GCCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGA120.10124873439082012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)