FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008093059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008093059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27871
Sequences flagged as poor quality0
Sequence length321
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA15545.575687991101862No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4971.7832155286857307No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4271.5320584119694307No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA4031.445947400523842No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3561.2773133364428975No Hit
GCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA3541.270137418822432No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3361.2055541602382405No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3221.1553227368949803No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3221.1553227368949803No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3211.1517347780847476No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3091.1086792723619532No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA3061.0979153959312546No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2971.065623766639159No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2951.0584478490186933No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA2941.0548598902084605No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2911.0440960137777617No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2660.9543970435219404No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2620.9400452082810089No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2600.9328692906605432No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2590.9292813318503103No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2550.9149294966093788No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.8862258261275161No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2470.8862258261275161No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2420.8682860320763517No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2400.8611101144558861No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2390.8575221556456532No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2370.8503462380251875No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2330.8359944027842561No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2290.8216425675433245No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2240.8037027734921603No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA2240.8037027734921603No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1980.710415844426106No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1840.6601844210828459No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1770.635068709411216No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1740.6243048329805174No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1710.6135409565498188No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1690.6063650389293531No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1670.5991891213088874No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1610.5776613684474902No Hit
ACCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1600.5740734096372574No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1590.5704854508270245No Hit
GCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1210.43414301603817584No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA1200.43055505722794307No Hit
CCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1190.42696709841771013No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1190.42696709841771013No Hit
TCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA1180.42337913960747736No Hit
ACCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA1120.4018513867460802No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA1100.39467546912561446No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA940.33726812816188867No Hit
TCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA890.31932833411072437No Hit
GCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA890.31932833411072437No Hit
TCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA810.2906246636288615No Hit
GCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA810.2906246636288615No Hit
ACCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA800.2870367048186287No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGA780.27986078719816293No Hit
CCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGA780.27986078719816293No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA750.2690969107674644No Hit
TCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA740.2655089519572315No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.2619209931469987No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA730.2619209931469987No Hit
ACCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA720.2583330343367658No Hit
GCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA710.25474507552653297No Hit
TCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA680.24398119909583438No Hit
ACCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA680.24398119909583438No Hit
ACCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA670.24039324028560152No Hit
GCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA670.24039324028560152No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGA660.23680528147536867No Hit
GCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA650.2332173226651358No Hit
TCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA650.2332173226651358No Hit
TCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.22962936385490296No Hit
TCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA640.22962936385490296No Hit
TCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA630.22604140504467007No Hit
GCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA620.22245344623443722No Hit
GCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA620.22245344623443722No Hit
GCCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGA620.22245344623443722No Hit
GCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA620.22245344623443722No Hit
ACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA610.21886548742420436No Hit
GCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA600.21527752861397154No Hit
GCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA600.21527752861397154No Hit
TCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA580.20810161099350577No Hit
ACCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA570.20451365218327294No Hit
CCCTATGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA560.2009256933730401No Hit
ACCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA560.2009256933730401No Hit
TCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA560.2009256933730401No Hit
TCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA550.19733773456280723No Hit
CCCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA550.19733773456280723No Hit
GCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA550.19733773456280723No Hit
TCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.19374977575257435No Hit
GCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA540.19374977575257435No Hit
ACCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA530.1901618169423415No Hit
ACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA520.18657385813210864No Hit
ACCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.18298589932187578No Hit
CCCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA510.18298589932187578No Hit
ACCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA500.17939794051164293No Hit
ACCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA490.17580998170141007No Hit
GCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA480.17222202289117722No Hit
CCCTATGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.17222202289117722No Hit
TCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.17222202289117722No Hit
ACCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA480.17222202289117722No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGA480.17222202289117722No Hit
ACCTACGGGGCGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA470.16863406408094433No Hit
ACCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA460.16504610527071148No Hit
TCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA460.16504610527071148No Hit
GCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA440.15787018765024577No Hit
TCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA430.1542822288400129No Hit
ACCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA430.1542822288400129No Hit
CCCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA420.15069427002978006No Hit
CCCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA420.15069427002978006No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGA420.15069427002978006No Hit
CCCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA410.1471063112195472No Hit
TCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.14351835240931435No Hit
GCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA400.14351835240931435No Hit
CCCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA400.14351835240931435No Hit
CCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.13993039359908147No Hit
GCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.13993039359908147No Hit
CCCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA390.13993039359908147No Hit
GCCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGA380.13634243478884864No Hit
TCCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA370.13275447597861575No Hit
CCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA370.13275447597861575No Hit
TCCTATGGGGCGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA360.1291665171683829No Hit
CCCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.1291665171683829No Hit
ACCTACGGGGTGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA360.1291665171683829No Hit
CCCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA350.12557855835815004No Hit
CCCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA350.12557855835815004No Hit
GCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.12557855835815004No Hit
CCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA350.12557855835815004No Hit
CCCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA350.12557855835815004No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA340.12199059954791719No Hit
ACCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA340.12199059954791719No Hit
TCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA340.12199059954791719No Hit
ACCTATGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA340.12199059954791719No Hit
CCCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.11481468192745148No Hit
TCCTATGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA320.11481468192745148No Hit
CCCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.11481468192745148No Hit
GCCTATGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA320.11481468192745148No Hit
TCCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGA300.10763876430698577No Hit
CCCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA300.10763876430698577No Hit
GCCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGA300.10763876430698577No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGA290.10405080549675289No Hit
TCCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGA290.10405080549675289No Hit
GCCTACGGGGCGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGA290.10405080549675289No Hit
GCCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGA280.10046284668652004No Hit
TCCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGA280.10046284668652004No Hit
GCCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGA280.10046284668652004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)