FastQCFastQC Report
Fri 10 May 2024
EGAF00008093172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008093172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319754765
Total Bases47.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC17613160.5508333863296767Illumina Single End PCR Primer 1 (100% over 50bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG4371990.13672947141225558Illumina Single End PCR Primer 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)