FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008094301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008094301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942566
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAAATAGGATTATCC37320.3959404434278342No Hit
GTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTAATGTGGTGGGTGAGTG32790.34788014844583826No Hit
GTTTTTTCATAGGAGGTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATG29940.31764353902007925No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG24850.26364201551933764No Hit
GTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTT20980.22258388272014903No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA19910.21123189251468863No Hit
GGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTGCA18650.19786412834751094No Hit
GTTCAAACTGTCATTTTATTTTTACGTTGTTAGATATGGGGAGTAGTGTG17410.1847085509131456No Hit
GATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTG17060.1809952830889296No Hit
GTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTC16700.1771759218983074No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG15880.16847626585300127No Hit
GTTTTTTGGAAAGTCATGTCAGTGGTAGTAATATAATTGTTGGGACGATT15390.16327769089909885No Hit
GTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGA14740.15638162208269765No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG14640.15532068841863594No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC14190.15054648693035821No Hit
GTTTTTTGTGAATTCTTCGATAATGGCCCATTTGGGCAAAAAGCCGGTTA13940.14789415277020387No Hit
AAAAATCCTGCGAAGAAAAAAACTTCTGAGGTAATAAATAGGATTATCCC13790.14630275227411133No Hit
GTTTGATTAGTCATTGTTGGGTGGTGATTAGTCGGTTGTTGATGAGATAT13570.1439686982131755No Hit
GTTTAATCCACCTCAACTGCCTGCTATGATGGATAAGATTGAGAGAGTGA13450.14269557781630146No Hit
GTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGTGCCTTGG13020.13813356306083605No Hit
CGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTGC12750.13526904216786942No Hit
GTTTTATGTTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTA12740.13516294880146323No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA12680.1345263886030262No Hit
GTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGGTGATGGTAGATGTGGC12660.13431420187021387No Hit
GGCATTTTTAATCTTAGAGCGAAAGCCTATAATCACTGCGCCCGCTCATA12260.13007046721396698No Hit
GTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGAC11740.12455361216084603No Hit
GGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT11680.123917051962409No Hit
GTTTTTTTCGTGATAGTGGTTCACTGGATAAGTGGCGTTGGCTTGCCATG11410.12105253106944236No Hit
GTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG10660.11309552858897944No Hit
GTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAG10580.11224678165773005No Hit
GTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCGTAGGT10570.1121406882913239No Hit
GTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAG10460.110973661260856No Hit
GTCAACGTCAAGGAGTCGCAGGTCGCCTGGTTCTAGGAATAATGGGGGAA10330.10959444749757577No Hit
CTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGAAA10330.10959444749757577No Hit
GCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC10240.10863960719992023No Hit
GTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTGTTAGATATGGGG10060.10672992660460913No Hit
GTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGG10030.10641164650539062No Hit
GGGAAATAGAATGATCAGTACTGCGGCGGGTAGGCCTAGGATTGTGGGGG9990.10598727303976592No Hit
CAAAAAGCTATTAGTGGGAGTAGAGTTTGAAGTCCTTGAGAGAGGATTAT9970.10577508630695356No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA9960.10566899294054741No Hit
GTTTGAAGTCCTTGAGAGAGGATTATGATGCGACTGTGAGTGCGTTCGTA9900.10503243274211037No Hit
TGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGT9830.10428977917726716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)