FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008094744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008094744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210427104
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA6658784131.6441369644093No Hit
TTGTTGAT5328215125.320954376675736No Hit
GCTCAACC5005948923.78946820462824No Hit
CAAAGTGG3686657217.51987804764922ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGTTGAT5223640.24823988453502643No Hit
GCTCAACA4029880.19150955002450637No Hit
NGCGCCTA3372220.16025597158814675No Hit
CAAAGTGA3025030.1437566711938401No Hit
NTGTTGAT2711160.12884081700805997No Hit
AGCGCCAA2679840.12735241558996127No Hit
NCTCAACC2531190.12028821154141817No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)