FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008094747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008094747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212815158
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA6666694931.32622207295967No Hit
TTGTTGAT5443785325.579875753023195No Hit
GCTCAACC5103348523.980192707889728No Hit
CAAAGTGG3723798817.497808121355718ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GCTCAACA4131370.19412949898991688No Hit
GTGTTGAT3905090.18349679772340277No Hit
NGCGCCTA3404710.1599843747972125No Hit
CAAAGTGA3067180.14412413236091012No Hit
NTGTTGAT2783020.1307717000120828No Hit
NCTCAACC2594400.12190860953616847No Hit
AGCGCCAA2398580.11270719729465886No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)