FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008094750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008094750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210018181
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA6631565931.57615149518889No Hit
TTGTTGAT5307692425.272537714246752No Hit
GCTCAACC5007284623.842148218586846No Hit
CAAAGTGG3685409817.54805123276446ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGTTGAT4998410.23799891876979928No Hit
GCTCAACA4056900.19316899045040295No Hit
NGCGCCTA3379600.16091940154457388No Hit
CAAAGTGA3033640.14444654198771487No Hit
NTGTTGAT2705210.12880837207136842No Hit
NCTCAACC2554390.12162708903759147No Hit
AGCGCCAA2529500.12044195354686935No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)