FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008094753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008094753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210843305
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTA6641187431.498213329562446No Hit
TTGTTGAT5344098625.346304451070907No Hit
GCTCAACC5028230923.848188587254405No Hit
CAAAGTGG3698016017.539167297723775ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGTTGAT4649850.22053581449977744No Hit
GCTCAACA4074320.1932392399180045No Hit
NGCGCCTA3341970.15850491434859648No Hit
CAAAGTGA3030630.14373849812304926No Hit
NTGTTGAT2710450.12855281318987102No Hit
NCTCAACC2532350.12010578187436399No Hit
AGCGCCAA2516140.11933696448175103No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)