FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008096347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008096347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72786214
Sequences flagged as poor quality0
Sequence length35-143
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5660327377.76647511848878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4506270.61911037164263No Hit
CGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCGTAGGGTGTAGATCTCGG816120.1121256286252229Illumina Single End PCR Primer 1 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)