FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429427
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG164353.8271929804134346No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA142723.3234985224496834No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT117722.7413273967403073No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA108172.518938026719326No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT98492.2935213668446557No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC96312.2427560446827983No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC90052.0969803948051706No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG87152.029448544222883No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT84481.9672726679971215No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT81101.8885631318012142No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC75981.7693344852559343No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA73011.7001725555216605No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA72051.6778171842944203No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTAGTGAGTAT71171.6573247606694503No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG69791.625188914530293No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA68401.5928201999408513No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT65441.5238911386568612No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT64841.5099190316398363No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG63391.4761531063486926No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC62431.4537977351214526No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT61471.4314423638942124No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA59101.3762525411769637No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA58071.3522670907977374No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA56381.3129123226997836No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG54561.2705302647481411No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT53731.2512021833745899No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA53031.2349013918547274No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG51521.199738255861881No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC51031.1883277017979774No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC49241.146644249197186No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG47881.114974139958596No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT47841.114042666157461No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC46661.0865641890239786No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT45481.059085711890496No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGCTAATG45211.0527982637328346No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC44351.0327715770084323No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG42840.9976084410155859No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG42430.9880608345539521No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG41380.9636096472741584No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG37300.8685993195583882No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC34820.8108479438880182No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA33630.7831365983042519No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG32020.7456447778085682No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA29150.6788115325771319No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT27290.6354980008243544No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT24750.5763494144522817No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC23770.5535283063244743No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGCTAGTGAGTATCTCG12230.2847981146970265No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTAATGAGTTT12060.28083935104220276No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG11140.2594154536160977No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG9050.21074594750679393No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGCTAGTGA6640.15462465098841013No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGCTAGTGAGTATCT6590.15346030873699137No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG6390.1488029397313164No Hit
TTTCGCTTCCCACAGGTCTCTGTACCGTACGGAAGGCAGTCTGGCTGAGA5920.13785812256798013No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA5000.11643422514187511No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGAGGGTGGGAGGCTG4870.11340693528818634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)