FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338688
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTAT160754.746256141345427No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA75812.238343253968254No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA70052.0682752267573696No Hit
TCCAAATACTCCACACGCAAAAATCCGTTGACCTCATGGTGGGGGCAGCA69292.0458356953892665No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT64491.9041123393801966No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC62441.8435846560846563No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA57281.6912320483749057No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC56421.665839947089947No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA54981.6233229402872262No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA49371.457683767951625No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC48041.4184145880574452No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTATCTCG45291.3372189153439153No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA41011.2108489229024944No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA38591.139396730914588No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG33530.9899966931216931No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA33150.9787769274376418No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA29930.8837041761148905No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC29100.8591978458049886No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC29060.8580168178382465No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC28960.8550642479213907No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTTAAAACGCTTGCAAATATCTTC25950.7661918934240364No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA25930.7656013794406651No Hit
CCCCTGCTCCTATAGCCAAGTCTGATGCCGACGAGTACCTCATAGACCAA24770.7313515684051398No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG23710.7000543272864701No Hit
TGTTCATTCATGATCCCACTGCTACCGCAAGTGTGAGTGACTGGTGGAGA22840.6743669690098262No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA20750.6126582577475435No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA20430.6032100340136054No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC20130.5943523242630385No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG20100.5934665532879819No Hit
CAGCCTCTGGCATTCTGGCAAGTTTGTACAAAAGAGCAGTCAGAGGACCA19510.5760463907785336No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA18290.5400250377928949No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA17850.5270337301587302No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG17790.5252621882086168No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG17630.5205380763416477No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG17210.5081372826908541No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGAGCGTG17090.5045941987906273No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT17080.5042989417989417No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT16390.48392620937263797No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG16320.48185941043083896No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG15770.465620275888133No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT15450.45617205215419504No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT14580.43048469387755095No Hit
CAGAGGGGAAAAATATGACAAAGTCTCGTGCTAAAATGGAGATTCTCTGT14560.4298941798941799No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACAAGAGGGATAAAACACCATGA14020.41395030234315944No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC13800.4074546485260771No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA13310.39298705593348454No Hit
TCAAATCATCCATTGCTTGGGTAGCAGAAAGTCAGTCCCATGGAGACCAA13210.3900344860166289No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT12980.38324357520786095No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACGCC12940.38206254724111866No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC12790.3776336923658352No Hit
CAGCCTCTGGCATTCTGGATCAGAGCAGTCAGAGGACCAGGAGACCAAGT12380.36552815570672714No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT12280.36257558578987154No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12050.35578467498110355No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG12000.3543083900226758No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT11560.34131708238851094No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGTGCAGATAATGACAAGGAATA11550.3410218253968254No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTAT11550.3410218253968254No Hit
GTGTCACTCGTAATTAGGTCCAGACGCTAATGAGTTTGTACTGAGGCCAG11440.3377739984882842No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG11400.33659297052154197No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT11300.33364040060468636No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATGA11130.32862103174603174No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA10810.31917280801209374No Hit
TGTGCTGTGACTGCTTGTAGGGTAACAGAGTTGAAAGTCAGGGCAAGACC10040.2964380196523054No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA9960.2940759637188209No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTATGAGCGTG9360.2763605442176871No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG9270.273703231292517No Hit
TCTTAAATGGCTACGACCCACACTGACGACATGGTTCTACATCTTAAATG9190.2713411753590325No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG8790.25953089569161No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT8600.2539210128495843No Hit
GCCTGCTGAAAATTACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA8430.2489016439909297No Hit
TTCTTATTCTGAGGTTATCTTTTTACCAGGACCAGGGACGAACTGGTGTA8320.24565381708238854No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATGA8240.24329176114890402No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA8120.23974867724867724No Hit
GTGTCACTCGTAATTAGGTCCATGTCGGCTAATGAGTTTGTACTGAGGCC7740.22852891156462585No Hit
TCCACAAACAGAACAAGATGCTGTACCGTAATCCTGTTTGAAGGAAAAGG7740.22852891156462585No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC7450.21996645880574453No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATGAGCGTGCTATCT7270.21465183295540438No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG6910.2040225812547241No Hit
GGAATCCTATGGCTTTCCAACCGACACTGACGACATGGTTCTACAGCAAC6750.1992984693877551No Hit
TGTGCTGTGACTGCTTGTAGACTGACGACATGGTTCTACAGAGTTGAAAG6410.18925973167044596No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT6350.1874881897203326No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG6330.18689767573696145No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCAACAATACGGCC6250.18453561980347694No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGAG6170.18217356386999245No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATGAGCG6090.17981150793650794No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT5990.1768589380196523No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG5860.17302059712773998No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG5750.1697727702191988No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTATCTCG5590.1650486583522298No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTT5570.16445814436885864No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGA5530.1632771164021164No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG5510.16268660241874527No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATGAGCGTGCTAT5490.16209608843537415No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA5410.15973403250188964No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA5350.15796249055177627No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA5220.15412414965986393No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTAGTGCTAAAATGGAGATTC5130.15146683673469388No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACGTGCTAAAATGGAGATT4940.14585695389266817No Hit
AGGACAAAATGTTTCACTTTTGGGTGCAGATAATGACAAGGAATATCTAG4900.14467592592592593No Hit
TGTGCTGTGACTGCTTGTACACTGACGACATGGTTCTACACAGAGTTGAA4820.14231386999244142No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTA4770.1408375850340136No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG4730.13965655706727134No Hit
GGTCTTCTCTGTTTCAGGGCATGGTAGATGTACACTGACGACATGGTTCT4540.13404667422524566No Hit
CAGAGGGGAAAAATATGACAAAGTGCTAAAATGGAGATTCTCTGTTAGAC4500.1328656462585034No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT4460.13168461829176115No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGGAAGGCAGTCTGGCTGAGACCA4340.1281415343915344No Hit
GGGGTCAGAGGCAAGCAGGACATGTAGGAGATAACACAGGCCCAAGAGAC4310.1272557634164777No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGACGTCAGTGCAGCCGTTACCTG4200.1240079365079365No Hit
TGTGCTGTGACTGCTTGTAGACTGACGACATGGTTCTACAGCAACAGAGT4180.12341742252456538No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATGAGCGTGCTAT4140.12223639455782313No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATG4100.12105536659108088No Hit
GAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGAGCGTGCT4070.1201695956160242No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG4020.11869331065759638No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC3970.11721702569916854No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGAG3940.11633125472411186No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA3940.11633125472411186No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATGA3890.11485496976568405No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC3870.11426445578231292No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTATGA3850.11367394179894179No Hit
AGCTGCTCACCATCGCTACGTAGCAGGTCTTGGCCAGTTGGAGACCAAGT3740.11042611489040062No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTTCAT3630.10717828798185941No Hit
TCTTAAATGGCTACGACCCAGACTGACGACATGGTTCTACATGGCTACCT3400.10038737717309146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)