FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338688
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG160794.747437169312169No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT104993.099903155706727No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA86952.5672595427059712No Hit
GCTGCCCCCACCATGAGGTCAACGGATTTTTGCGTGTGGAGTATTTGGAT67681.9982993197278913No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC65841.9439720332577477No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA53541.580805933484505No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA51681.5258881330309901No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC45211.3348568594104309Illumina Single End PCR Primer 1 (100% over 21bp)
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA44601.3168461829176115No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA42641.2589758125472412No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA40831.205534297052154No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC39041.1526832955404385No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA34001.0038737717309145No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC32750.9669666477702192No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG32400.9566326530612245No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC30700.9064389644746788No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG30640.9046674225245653No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA29320.865693499622071No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG27850.8222907218442932No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA27660.8166808390022675No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA27470.8110709561602418No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT23980.7080262660619803No Hit
CCACCAGTCACTCACACTTGCGGTAGCAGTGGGATCATGAATGAACATGT22920.6767290249433107No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA22810.6734811980347695No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT22790.6728906840513984No Hit
CCTGGTCCTCTGACTGCTCTTTTGTACAAACTTGCCAGAATGCCAGAGGC19910.5878566704459562No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG19810.5849041005291006No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC18500.5462254346182918No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC18360.5420918367346939No Hit
ATGAGGTACTCGTCGGCATCAGACTTGGCTATAGGAGCAGGGGTGTAGAA17120.5054799697656841No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT17030.502822656840514No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT16760.4948507180650038No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG16620.49071712018140584No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCGTTTTAACTATCAAGAAAAAC16410.48451672335600904No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG16310.48156415343915343No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC15930.47034438775510207No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT15540.4588293650793651No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG15010.4431807445200302No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT14290.42192224111866966No Hit
CCATGGGACTGACTTTCTGCTACCCAAGCAATGGATGATTTGATGTAGAA13210.3900344860166289No Hit
AACAGAGAATCTCCATTTTAGCACGAGACTTTGTCATATTTTTCCCCTCT13170.38885345804988664No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG13150.38826294406651546No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA12700.3749763794406652No Hit
CCTGGTCCTCTGACTGCTCTGATCCAGAATGCCAGAGGCTGTGTAGAACC12610.3723190665154951No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC12050.35578467498110355No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT12030.35519416099773243No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT12020.35489890400604684No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT11880.350765306122449No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC11740.3466317082388511No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT11650.343974395313681No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG11570.34161233938019653No Hit
GGCCTCAGTACAAACTCATTAGCGTCTGGACCTAATTACGAGTGACACTG11290.3333451436130007No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA10850.320353835978836No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA10830.3197633219954648No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTACCCAAAAGTGAAACATTT10480.30942932728647016No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA9920.2928949357520786No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT9700.2863992819349962No Hit
TGCCCTGACTTTCAACTCTGTTACCCTACAAGCAGTCACAGCACATGTAG9500.280494142101285No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC9370.2766558012093726No Hit
TTCATGGTGTTTTATCCCTCTTGTCTTTCATGGATTTGACGGCTCCTCTA9190.2713411753590325No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA8980.2651407785336357No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC8550.25244472789115646No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC8450.24949215797430083No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA8400.248015873015873No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG8390.24772061602418743No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA8210.2424059901738473No Hit
GGCCTCAGTACAAACTCATTAGCCGACATGGACCTAATTACGAGTGACAC7700.22734788359788358No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC7600.22439531368102797No Hit
TCATTACACCAGTTCGTCCCTGGTCCTGGTAAAAAGATAACCTCAGAATA7570.2235095427059713No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTACAGCATCTTGTTCTGTTTGTG7460.2202617157974301No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC7360.21730914588057446No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTCGGTTGGAAAGC6970.2057941232048375No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG6960.20549886621315192No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA6620.19546012849584277No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT6390.18866921768707481No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC6070.17922099395313681No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGCAGGCTGTAGAACCATGTCGTC5900.17420162509448223No Hit
CCACCAGTCACTCACACTTGACCATAATCATGTAGACATCGATGGTACAT5800.17124905517762662No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA5700.16829648526077098No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA5650.16682020030234318No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5650.16682020030234318Illumina Single End PCR Primer 1 (95% over 22bp)
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG5590.1650486583522298No Hit
TGCCCTGACTTTCAACTCTGTAGAACCATGTCGTCAGTCTACAAGCAGTC5540.16357237339380196No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT5430.16032454648526076No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT5370.1585530045351474No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG5130.15146683673469388No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT4980.14703798185941044No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG4900.14467592592592593No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC4830.14260912698412698No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGT4750.1402470710506425No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG4660.13758975812547242No Hit
AACAGAGAATCTCCATTTTAGCACTAGCAGAGACTTTGTCATATTTTTCC4580.1352277021919879No Hit
AACAGAGAATCTCCATTTTAGCACGTAGCAGAGACTTTGTCATATTTTTC4500.1328656462585034No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCAAAAGTGAAACATTTTGTCC4420.1305035903250189No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA4320.12755102040816327No Hit
CAGCCAGACTGCCTTCCGGTAGCAGAGACCTGTGGGAAGCGAAATGTAGA4300.12696050642479215No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTACAAGCAG4240.12518896447467875No Hit
CTTGGGCCTGTGTTATCTCCTACATGTCCTGCTTGCCTCTGACCCCTGTA4190.12371267951625095No Hit
AACAGAGAATCTCCATTTTAGCACTTTGTCATATTTTTCCCCTCTGTGTA4140.12223639455782313No Hit
CACACACAGGTAACGGCTGCACTGACGTCTACATGCCTTTAACTGTCAAA4100.12105536659108088No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4070.1201695956160242No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC3980.11751228269085412No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG3950.11662651171579744No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG3890.11485496976568405No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT3760.11101662887377173No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA3760.11101662887377173No Hit
CCAACTGGCCAAGACCTGCTACGTAGCGATGGTGAGCAGCTTGTAGAACC3740.11042611489040062No Hit
CTTGGGCCTGTGTTATCTCCTCCTGCTTGCCTCTGACCCCTGTAGAACCA3710.10954034391534391No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT3690.10894982993197277No Hit
TGCCCTGACTTTCAACTCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT3630.10717828798185941No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTCTACAA3560.10511148904006047No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT3490.10304469009826153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)