FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660778
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC268814.068083380499956No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC187692.8404396030134174No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC175672.658532820402616No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA165882.5103741347320887No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG144622.188632188117643No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG127911.9357484662019622No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC120241.8196731731383309No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG118301.7903138421678688No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA112411.7011764919534247No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG111971.6945176746199178No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC105731.6000835378901839No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA104871.58706857673833No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC104271.5779883712835476No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA98611.4923317664934366No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT93461.4143933363398902No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA92871.4054644676426877No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT91631.386698709702805No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT89111.34856184679272No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG88751.343113723519851No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG86071.302555472488491No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT82751.25231166897203No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT80111.2123587649709888No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC79681.2058512843950617No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT78361.185874832394541No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA76571.1587855527877744No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT73711.1155032401199798No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT71491.0819064799372862No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT71021.0747936523310402No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT70781.0711615701491273No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT69301.0487637300273314No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT67491.0213717769054054No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA64800.9806621891164657No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC63140.9555402873582354No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA57590.8715483869015008No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG53950.8164618071424896No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT51770.7834703939901146No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT51370.7774169236869266No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT51220.7751468723232311No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT49420.7479062559588848No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT45460.687976899957323No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC43530.6587689057444407No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT41500.6280475439557612No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT40040.6059523773491249No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG39350.5955101410761254No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC39190.5930887529548502No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC37230.5634267484692287No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC36830.5573732781660407No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC33320.5042540762555654No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA29950.45325358895120604No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT29540.44704878189043823No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC21580.32658472285699586Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG14980.22670246285439286No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA14190.21474685900559642No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC12510.18932228373220658No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA11660.17645865933793195No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG10010.15148809433728122No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG9730.14725066512504956No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT9290.1405918477915427No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA8970.13574907154899227No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG7870.11910202821522509No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT7160.10835711842706627No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC7150.10820578166948658No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA6740.10200097460871882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)