FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523654
Sequences flagged as poor quality0
Sequence length125
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACAT237204.529708548010709No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA137692.629407967856638No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA136242.6017179282503333No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC118712.266954897699626No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA109032.082100012603742No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA92671.7696799795284675No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA80511.5374655784162825No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG78721.5032827019367752No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC73391.4014979356598058No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC69621.3295038326834132No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC66141.2630477376282814No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACATCTCG62201.1878072162152873No Hit
TGACAGTTTGACAGTTAAAGGCATCACTGACGACATGGTTCTTGACAGCC51940.9918763152768814No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA50480.9639953098801881No Hit
GGTCTTCTCTGTTTCAGGGCATACACTGACGACATGGTTCTACAGCAACA48620.9284756728679624No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA47590.9088061964579666No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGGTGCTAAAATGGAGA45000.8593460567473944No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC44420.8482700409048723No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA38930.7434298219816903No Hit
GGAATCCTATGGCTTTCCAACTACACTGACGACATGGTTCTACAGCAACA37030.7071463218079113No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA36740.7016083138866503No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG36320.6935877506903413No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT35180.671817650586074No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA34870.6658977110840364No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA34700.6626512926474352No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTACTGGGTGCGGA34350.6559674899838444No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC33910.6475649952067586No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG33300.6359160819930718No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG33170.6334335267180237No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGGCTAATGAGTTTGTACTGAGG32400.6187291608581238No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC31970.6105176318714265No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT31620.6038338292078357No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG30580.5839733870074515No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT30520.5828275922651217No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG30460.5816817975227918No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG29440.5622032869031842No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTATGAGC28360.5415789815412467No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG28240.5392873920565869No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT28140.537377734152704No Hit
GACCCAAAACCCAAAATGGCTACGACCCAGTACGGTAGGAGACAGAAGCA27120.5178992235330963No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC26070.4978478155423237No Hit
TACCAGGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTATGAGCA24540.4686300496129123No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTGTGACA24420.4663384601282526No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT24200.46213721273970987No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG22980.438839386312336No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG22390.42757240467942575No Hit
GACCCAAAACCCAAAATGGCCAATCTGAGACCAAGTCTCTGCTACCGTAG20470.39090697292487026No Hit
GCAACAGATAACTCAGATTGCCTTCACTGACGACATGGTTCTACATACCA19980.38154964919584305No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA18820.35939761751079913No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAGGATGCTGAGG18810.35920665172041083No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG18700.3571060280261394No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA18350.3504222253625486No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG17720.33839138056808504No Hit
TGTGCTGTGACTGCTTGTAGGGTAGCACAGAGTTGAAAGTCAGGGCAAGA17460.33342627001798897No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACAT17410.3324714410660474No Hit
CAGAGGGGAAAAATATGACAAATACGGTGCTAAAATGGAGATTCTCTGTT16020.30592719620207237No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTA15170.289695104019066No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG14970.28587578821129983No Hit
ACTTCTACCGTGCCCTGTGTCTACTCATTTGAACAGACCAAGTCTCTGCT14740.2814835750323687No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG14700.28071971187081546No Hit
AGATGAGTCATATTTGTGGGTTTTCAACACTGACGACCACTGACTCTGAT14590.27861908817654407No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG13820.2639147223166442No Hit
TCAAATCATCCATTGCTTGGGGTAGCAGAAAGTCAGTCCCATGGAGACCA13380.25551222753955855No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTACACTGACGACATGGTTCTACA13310.25417546700684035No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG13250.2530296722645105No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT13040.249019390666356No Hit
TCCCTGCCAGCGAGATGATGCAGAGGCCTTTGCATCCCTAGACCAAGTCT12740.24329041695470674No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCCCTGCTACCGTAGCTAATG12340.23565178533917433No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTA12240.23374212743529124No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA12070.23049570899868999No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT11880.22686735898131208No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG11810.22553059844859394No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCAAGAGGGATAAAACA11710.2236209405447108No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCAAGTCTACCACTGACTCT11450.21865582999461478No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT11440.21846486420422645No Hit
ATACGGCCAGGCATTGAAGTGACACTGACGACAGGGGCCAGGAGGAGACC11260.21502747997723687No Hit
GGATGCAGAGCTTCTTCCCACACTGACGACATGGTTCTACAGGATGCAGA11100.21197202733102394No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTATGAGCACATCT10830.20681595099053957No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAGTATGA10240.19554896935762928No Hit
GGGACGAACTGGTGTAATGAAGACCAAGTCTCTGCTACCGTAGTATGAGC10220.19516703777685265No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT9760.1863826114189904No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACA9600.18332715877277744No Hit
TCTTAAATGGCTACGACCCAGGACGACATGGTTCTACATCTTAAATGGCT9600.18332715877277744No Hit
CTGGTGTTGTTGGGCAGTGTCTATCTCCCCTTTCTTGCGGAGATAGACCA9210.17587949294763336No Hit
GCCTGCTGAAAATTACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA9170.1751156297860801No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA9140.1745427324149152No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTAT9070.17320597188219702No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG8850.1690047244936542No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCCTTCCACTCGGATAAGATGCTA8830.16862279291287757No Hit
TGTGCTGTGACTGCTTGTAGGGTAACAGAGTTGAAAGTCAGGGCAAGACC8800.16804989554171265No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTAGTATGAGCACAT8630.1648034771051114No Hit
TCTTAAATGGCTACGACCCACACTGACGACATGGTTCTACATCTTAAATG8480.16193899024928676No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACAGCAACAGAGGAG8430.1609841612973452No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG8050.1537274612625894No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGGGTGGGGGTTGTTGACTTG8040.1535364954722011No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT8000.15277263231064786No Hit
TCATAATGCTTGCTCTGATAGGAGTACACTGACGACATGGAGTGGGTCCC7890.15067200861637647No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACATCTCG7840.1497171796644349No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCTACGGAAGGCAGTCTGGCTGAGA7830.1495262138740466No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCAAGTACCACTGACTCTGA7740.1478075217605518No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCGTGGACAAC7720.1474255901797752No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGCAACAGG7530.1437972401623973No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAGTATGAGCACAT7490.14303337700084406No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT7440.14207854804890252No Hit
TCCACAAACAGAACAAGATGCTGTAGAACCAAATCCTGTTTGAAGGAAAA7400.14131468488734927No Hit
GGGGTCAGAGGCAAGCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCA7400.14131468488734927No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC7380.14093275330657268No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTAT7240.13825923224113632No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA6950.13272122431987535No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC6770.12928384009288577No Hit
ATACGGCCAGGCATTGAAGTCATGGTTCGACAGGGGCCAGGAGGAGACCA6740.12871094272172082No Hit
ATACGGCCAGGCATTGAAGTACCATGGACAGGGGCCAGGAGGAGACCAAG6620.1264193532370611No Hit
TCCACAAACAGAACAAGATGCTTCTACCGTAATCCTGTTTGAAGGAAAAG6490.12393679796201308No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACATGGTTCGACAGGGGCCAGG6470.12355486638123646No Hit
TGTGGTCTGCCAGCTAAAGGGTTTGTACTTTACTTTCATTGGGAGAAGAC6150.11744396108881056No Hit
AGAAAACGGCATTTTGAGTGTGTACTGAGGCATAACTGCACCCTTAGACC6130.11706202950803393No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGTATGAG6110.11668009792725731No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGTA6020.11496140581376253No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG5940.11343367949065604No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA5700.1088505005213366No Hit
ATACGGCCAGGCATTGAAGTGTACACTGACGACAGGGGCCAGGAGGAGAC5440.10388538997124054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)