FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523654
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG238864.561408869215168No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT191363.6543213648706967No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA133082.5413727384876275No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC128912.4617400038957022No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG100141.9123314249485348No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA92731.770825774270797No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA87981.6801170238363499No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC81131.5493054574203577No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA65631.2533084823184775No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA63571.2139695294984856No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC63051.2040393083982934Illumina Single End PCR Primer 1 (100% over 21bp)
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT50460.9636133782994114No Hit
CACACACAGGTAACGGCTGTCAAGAACCATGTCGTCAGTGATGCCTTTAA50240.9594121309108685No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG48370.9237015281082548No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA48200.9204551096716534No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG43280.826499940800605No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA42330.8083581907137155No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGTTGGAAAGCC38260.7306351140256734No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT37740.7207048929254813No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC36530.6975980322884958No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA35450.6769737269265583No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTGGGAAGAAGCTCT33440.6385896030585081No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC32790.6261768266832679No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA32200.6149098450503576No Hit
GGCCTCAGTACAAACTCATTAGCCTTGGTCTGGACCTAATTACGAGTGAC32160.6141459818888044No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA31970.6105176318714265No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC31620.6038338292078357No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTTGTCATATTTT31100.5939036081076436No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC30900.5900842922998774No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG30770.5876017370248293No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC30450.5814908317324035No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG29090.5555194842395933No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT26510.5062503103194094No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG26320.5026219603020315No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT25670.49020918392679136No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG25320.48352538126320055No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT25280.4827615181016473No Hit
TCCCTGCTTCTGTCTCCTACCGTACTGGGTCGTAGCCATTTTGGGTTTTG24420.4663384601282526No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT24250.46309204169165136No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT22520.43005495995447374No Hit
GTTTCCTCTGGTCCTGGTATGTAGAACCATGTCGTCAGTGTAGATCTCGG22010.42031570464466994No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTCTCG21010.401219125605839No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG19990.38174061498623135No Hit
GTTTCCTCTGGTCCTGGTATGTAGAACCATGTCGTCAGTGAAGGCAATCT19630.3748658465322522No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG18690.3569150622357511No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT18500.3532867122183732No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG17930.3424016621662395No Hit
CAGATTGGCCATTTTGGGTTTTGGGTCTGTAGAACCATGTCGTCAGTGTA17700.3380094489873084No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG17350.3313256463237176No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT16770.3202496304811956No Hit
TGCCCTGACTTTCAACTCTGTGCTACCCTACAAGCAGTCACAGCACATGT16670.3183399725773125No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA15750.300771119861588No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA15180.2898860698094543No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA14960.28568482242091153No Hit
AACAGAGAATCTCCATTTTAGCACCGTATTTGTCATATTTTTCCCCTCTG14580.2784281223861557No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT14390.27479977236877784No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC14140.2700256276090701No Hit
GTTCAAATGAGTAGACACAGGGCACGGTAGAAGTTGTAGAACCATGTCGT14100.26926176444751687No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACACTACTCCCCTG13570.2591405775569365No Hit
CCATGGGACTGACTTTCTGCTACCCCAAGCAATGGATGATTTGATGTAGA13420.2562760907011118No Hit
TCTGGATCAGAGTCAGTGGTCGTCAGTGTTGAAAACCCACAAATATGACT12960.24749166434324954No Hit
AGGGATGCAAAGGCCTCTGCATCATCTCGCTGGCAGGGATGTAGAACCAT12810.24462717748742488No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA12420.2371795116622808No Hit
GGCCTCAGTACAAACTCATTAGCTACGGTAGCAGGGACTTGGTCTGGACC12140.23183246953140815No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGACCCCTGTAGAACCATGTCGT11600.22152031685043944No Hit
CCTCCTGGCCCCTGTCGTCAGTGTCACTTCAATGCCTGGCCGTATTGTAG11320.21617327471956674No Hit
TCTGGATCAGAGTCAGTGGTAGACTTGGTCTATGAAAACCCACAAATATG10060.1921115851306397No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG9960.1902019272267566No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC9780.18676454299976702No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC9770.18657357720937873No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC9640.1840910219343307No Hit
AACAGAGAATCTCCATTTTAGCACCGGTAGCAGAGACTTGGTCTAACAGA9580.18294522719200082No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG9450.18046267191695278No Hit
TCATTACACCAGTTCGTCCCTGTAGAACCATGTCGTCAGTGTAGATCTCG9320.17798011664190477No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT9180.17530659557646844No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTATCTCGCTGGCA9090.17358790346297365No Hit
AGCATCTTATCCGAGTGGAAGGGTACGGTAGCAGTAGGGGGGCTTTCTCT9070.17320597188219702No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC8500.16232092183006336No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT8370.15983836655501532No Hit
TGCCCTGACTTTCAACTCTGTTACCCTACAAGCAGTCACAGCACATGTAG8350.1594564349742387No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8350.1594564349742387No Hit
ATCTCCGCAAGAAAGGGGAGATAGACACTGCCCAACAACACCAGTGTAGA8340.1592654691838504No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG8200.15659194811841406No Hit
TTCATGGTGTTTTATCCCTCTTGACTTGGTCTTTCATGGATTTGACGGCT8190.15640098232802577No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC8010.15296359810103619No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT8000.15277263231064786No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTACTTCAATGC7940.15162683756831802No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC7930.15143587177792972Illumina Single End PCR Primer 1 (95% over 22bp)
CAGCCAGACTGCCTTCCGTAGGTAGCAGAGACCTGTGGGAAGCGAAATGT7910.1510539401971531No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTACTATCAAGAAA7780.14857138492210506No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTACTCCTATCAGAGCAAGCAT7760.14818945334132844No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7610.14532496648550378No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG7580.14475206911433885No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG7530.1437972401623973No Hit
ACAAGTCAACAACCCCCACCCACTGACTTCCATTTTCTACTTTTTCTGAG7420.1416966164681259No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGT7250.13845019803152464No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT7180.13711343749880647No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGCAGGCTGTAGAACCATGTCGTC7140.13634957433725323No Hit
CTTGGGCCTGTGTTATCTCCCCTCCTCTGCTTGCCTCTGACCCCTGTAGA7110.1357766769660883No Hit
GGCCTTTTCCTTCAAACAGGATTTGGTTCTACAGCATCTTGTTCTGTTTG7100.1355857111757No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT7010.1338670190622052No Hit
TCTGGATCAGAGTCAGTGGTACTTGGTCTATGAAAACCCACAAATATGAC6960.13291219011026364No Hit
CCTCCTGGCCCCTGTCGAACCATGACTTCAATGCCTGGCCGTATTGTAGA6870.13119349799676885No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC6820.1302386690448273No Hit
CCTCCTGGCCCCTGTCCATGGTACTTCAATGCCTGGCCGTATTGTAGAAC6810.13004770325443898No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG6690.12775611376977927No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACGTAGCGCCTCTG6580.1256554900755079No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGACTTCAATGCCTGGCCG6560.12527355849473126No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT6460.12336390059084815No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGAAGCATCTTGTTCTGTTTGT6390.12202714005813No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT6370.12164520847735337No Hit
AGGGATGCAAAGGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTA5910.11286078211949112No Hit
TCTCCCAATGAAAGTAAAGTACAAACCCTTTAGCTGGCAGACCACATGTA5900.1126698163291028No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC5890.1124788505387145No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA5890.1124788505387145No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA5770.11018726105405478No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAGTAATACACATAGCGCCTCTG5500.10503118471357042No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACACTTCAATGCCTGGCCGTATTGTA5410.10331249260007562No Hit
AAGGGTGCAGTTATGCCTCAGTACACACTCAAAATGCCGTTTTCTTGTAG5290.10102090311541591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)