FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362665
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC149104.111232128824121No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC95252.6263907462810034No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA94222.5979898804682007No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC86102.3740917927012535No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG77552.138337032798864No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG73842.0360387685605175No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC64901.7895302827678434No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG63841.7603022072711731No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG61191.687232018529497No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA58671.6177464050845822No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA53701.4807053341237781No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT53281.4691243985496256No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA52291.4418264789819806No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA50651.3966056829305282No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC48421.3351164297630045No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT48361.3334620103952683No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC47821.3185722360856438No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT45141.2446748376600996No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG43261.1928363641377029No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC42961.1845642672990224No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT41161.1349316862669405No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG41071.1324500572153364No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT39391.0861263149187266No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT39211.0811630568155186No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT36260.9998207712351619No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT35040.966180910757862No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT33700.9292322115450897No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA33620.9270263190547751No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT33390.9206843781451202No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT33130.9135152275515972No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT32930.9080004963258103No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT31460.8674672218162767No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC29880.8239008451325602No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG29670.8181103773454841No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA28610.7888823018488136No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA27080.746694607971544No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC26430.7287717314877367No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT24730.6818965160685481No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT22920.6319881984751768No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT21830.6019329132946384No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT20840.5746349937269932No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT20130.5550576978754498No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC18950.5225207836433072No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG18860.520039154591703No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT18530.5109398480691547No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA18270.5037706974756317No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA18030.4971530200046875No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC17140.4726124660499359No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT16530.45579253581128587No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC15810.43593950339845305No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC12820.3534942715729392Illumina Single End PCR Primer 1 (100% over 21bp)
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC12820.3534942715729392No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC11110.30634331959246136No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10910.3008285883666745No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG10250.28262997532157774No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA9540.2630526794700343No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7490.20652668440571878No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG6660.1836405498187032No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5880.16213309803813436No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA5700.15716983993492617No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT5640.15551542056719012No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA5610.15468821088332207No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT5470.15082789902527124No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA5440.15000068934140323No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG5260.14503743123819504No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT5220.14393448499303765No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC5210.1436587484317483No Hit
CCAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT5160.14228006562530157No Hit
CCAACTGGCCAAGACCTAGCGATGGTGAGCAGCTTGTAGAACCATGTCGT5140.1417285925027229No Hit
CCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT4660.1284932375608344No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA4340.11966966759957537No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT4330.11939393103828602No Hit
CAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT4200.11580935574152455No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT3640.10036810830932129No Hit
CTGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA3630.10009237174803193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)