FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59948
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT802713.389937946220057No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA50848.480683258824314No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG19663.279508907720024No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC18253.044305064389137No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG17292.8841662774404484No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC16462.7457129512243945No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT15692.6172682991926335No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG15192.5338626809901914No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA12542.0918129045172487No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC12122.021752185227197No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCGTATC8671.4462534196303463No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC8371.396210048708881No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT8171.3628478014279042No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC7401.2344031493961434No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA7301.217722025755655No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGC6461.0776005871755523No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG5610.9358110362314006No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT5130.8557416427570561No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACGCGTATCATATCT5060.8440648562087143No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA4720.7873490358310536No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA4320.7206245412690998No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT4150.6922666310802695No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG3510.5855074397811436No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG3370.5621538666844599No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT3000.5004337092146527No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT2790.465403349569627No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG2610.43537732701674786No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT2570.4287048775605525No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG2550.4253686528324548No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC2470.412023753920064No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG2470.412023753920064No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC2390.3986788550076733No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA2370.39534263027957567No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT2350.3920064055514779No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA2260.37699339427503836No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG2250.3753252819109895No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA2250.3753252819109895No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC2130.3553079335424034No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG2080.3469673717221592No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC2060.3436311469940615No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCG1940.3236137986254754No Hit
GGAAACCGTAGCTGCCCTGTTAAAGATTCATCTGGACCTGGGTCTTAGAC1840.306932674984987No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTACGC1710.28524721425235205No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACGCGTATCATAT1700.2835791018883032No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCG1680.2802428771602055No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG1670.27857476479615667No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTACGCGTA1640.2735704277040102No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA1540.25688930406352173No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG1460.24354440515113096No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT1460.24354440515113096No Hit
GGAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT1450.24187629278708214No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG1430.23854006805898448No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC1400.2335357309668379No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG1290.21518649496230063No Hit
TGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG1240.20684593314205646No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT1230.20517782077800759No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA1220.20350970841395877No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGC1200.20017348368586108No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT1180.1968372589577634No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG1170.19516914659371457No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACGC1120.18682858477347034No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC1080.18015613531727495No Hit
GCAACAGAGGAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT1050.17515179822512844No Hit
AGCTGCTCACCATCGCTACCGAGGTCTTGGCCAGTTGGAGACCAAGTCTC1040.1734836858610796No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG1020.1701474611329819No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA1010.16847934876893308No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA980.16347501167678655No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA980.16347501167678655No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG930.15513444985654234No Hit
NCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT920.1534663374924935No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG910.15179822512844465No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT900.1501301127643958No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTACGCGTATCATATCT850.1417895509441516No Hit
GGAATCCTATGGCTTTCCAACCCAGCAACAGAGGAGGGGGAGACCAAGTC820.1367852138520051No Hit
GCAACAGATGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT810.13511710148795625No Hit
ACAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT790.13178087675985853No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT780.13011276439580968No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG760.12677653966771202No Hit
AGCTGCTCACCATCGCTACGGTACAGGTCTTGGCCAGTTGGAGACCAAGT740.12344031493961433No Hit
GTAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT680.11343164075532128No Hit
CAGAGGGGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG660.11009541602722359No Hit
CAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG640.10675919129912591No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA630.10509107893507706No Hit
CGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG620.10342296657102823No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA610.10175485420697938No Hit
GCAACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCAT610.10175485420697938No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA610.10175485420697938No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG610.10175485420697938No Hit
AGCTGCTCACCATCGCTACGGAGCAGGTCTTGGCCAGTTGGAGACCAAGT600.10008674184293054No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG600.10008674184293054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)