FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008097177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008097177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192893
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA84164.363040649479244No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA62353.2323619830683334No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGAGAGACAAT58383.026548397297984No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA57772.9949246473433457No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT55752.8902033770017574No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC53932.7958505492682475No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC49442.5630790127168948No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT47982.487389381677925No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG44942.3297890540351385No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA42532.204849320607798No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA40002.073688521615611No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT37611.9497856324490779No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC33521.7377509811138818No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC33441.7336036040706506No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT30681.5905190960791733No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG29081.507571555214549No Hit
CAGAGGGGAAAAATATGACAAAGTCGTGCTAAAATGGAGATTCTCTGTTA25601.3271606538339908No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC24881.2898342604449098No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG24291.2592473547510796No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT23891.2385104695349236No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA23441.215181473666748No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC23221.203776186797862No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG22941.1892603671465527No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA21841.1322339328021234No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT21551.1171996910204103No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT18970.9834467813762034No Hit
TTTCGCTTCCCACAGGTCTCTGCTACGGAAGGCAGTCTGGCTGAGACCAA17790.9222729699885428No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA17480.9062018839460219No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT16470.8538412487752276No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG16180.8388070069935145No Hit
ATACGGCCAGGCATTGAAGTGTTCGACAGGGGCCAGGAGGAGACCAAGTC15750.8165148553861468No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG15140.7848911054315088No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA14760.7651910644761604No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG13850.7180146506094052No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC13780.7143856956965778No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG13370.693130388350018No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA13120.6801698350899203No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG12990.6734303473946696No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT12260.6355855318751846No Hit
TGACCAATGGCTAAGTGAAGATGAGACTTGTTTTTCCTTTCCTCTTCCTG12080.6262539335279144No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGTAATTTAGTGAAATAACTAT11690.6060354704421622No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT10860.5630064336186383No Hit
TGACAGTTTGACAGTTAAAGGCATGTCGTCAGCCGTTACCTGTGTGTGAG9990.5179037082734989No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTGAGAGACAATCTCG9980.5173852861430949No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG9910.5137563312302675No Hit
TGTGCTGTGACTGCTTGTAGAGTGTACAGAGTTGAAAGTCAGGGCAAGAC9640.49975893370936214No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTG9320.48316942553643727No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG9240.4790220484932061No Hit
TTTCGCTTCCCACAGGTCTCTACGGAAGGCAGTCTGGCTGAGACCAAGTC8750.45361936410341486No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG7980.4137008600623143No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG7960.4126640158015065No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTCTTTCTTCAGCCACAGGCTCCCAG6520.3380112290233446No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT6400.3317901634584977No Hit
GGGGTCAGAGGCAAGCAGGTGGCAGGAGATAACACAGGCCCAAGAGACCA5990.3105348561119377No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTGAGAGACAATCT5970.3094980118511299No Hit
GACCCAAAACCCAAAATGGCAGGAGAGGGAGAGATGGGGGTGGGAGGCTG5940.30794274545991823No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA5840.30275852415587917No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTGAGAGA5090.2638768643755865No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG4120.2135899177264079No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA4050.20996096281358062No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAGGGGCCAG3960.2052951636399455No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGTG3350.1736714136853074No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA3250.16848719238126839No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3020.1565634833819786No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA3010.1560450612515747No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGCGGTGGAT2900.15034241781713176No Hit
GCAGCTTCTGCCATCTCTCTCCCCCTTTTTCTTCAGCCACAGGCTCCCAG2830.14671346290430445No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAGTGAGA2770.14360293012188105No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA2610.1353081760354186No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA2460.12753184407936005No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG2410.12493973342734054No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGAGAGACAAT2380.12338446703612883No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTAGTGAGAGACAAT2380.12338446703612883No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1950.10109231542876101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)